biostudies-literatureUnknown20(1)Tzounakas VL<h4>Background</h4>Proteasomes are proteolytic complexes with prominent roles in the control of protein homeostasis and cellular viability. However, little is known about the effects of storage and glucose-6-phosphate dehydrogenase deficiency (G6PD^-) on the activity and topology of red blood cell (RBC) proteasomes.<h4>Materials and methods</h4>We investigated the concentration (by GeLC-MS proteomics analysis and immunoblotting), activity (by using peptide substrates and proteasome inhibitors), and subcellular/extracellular distribution (following cell fractionation and isolation of extracellular vesicles, respectively) of RBC proteasomes in fresh blood and RBCs from control and G6PD^- donors following storage in leukoreduced units. RBC proteasome activity was also testBlood transfusion = Trasfusione del sangue27-39https://www.ebi.ac.uk/biostudies/studies/S-EPMC8796840biostudies-literatureRed cell proteasome modulation by storage, redox metabolism and transfusion.PMC8796840Papassideri ISD'Alessandro AAnastasiadi ATDzieciatkowska MStamoulis KSiourounis PKriebardis AGVergaki AKaradimas DGTzounakas VLAntonelou MHfalseRed cell proteasome modulation by storage, redox metabolism and transfusion.<h4>Background</h4>Proteasomes are proteolytic complexes with prominent roles in the control of protein homeostasis and cellular viability. However, little is known about the effects of storage and glucose-6-phosphate dehydrogenase deficiency (G6PD^-) on the activity and topology of red blood cell (RBC) proteasomes.<h4>Materials and methods</h4>We investigated the concentration (by GeLC-MS proteomics analysis and immunoblotting), activity (by using peptide substrates and proteasome inhibitors), and subcellular/extracellular distribution (following cell fractionation and isolation of extracellular vesicles, respectively) of RBC proteasomes in fresh blood and RBCs from control and G6PD^- donors following storage in leukoreduced units. RBC proteasome activity was also test2022-01-01T00:00:00Z2022 Jan2025-04-04T07:45:28.872Z2025-04-04T07:45:28.872ZS-EPMC87968403326352110.2450/2020.0179-20