biostudies-literatureUnknown10(3)Yang Y<h4>Background</h4>We demonstrated that drinking hydrogen-rich water (HRW) inhibits endometrial tumor growth in our previous work. This research is to identify differentially expressed proteins (DEPs) between HRW and purified water groups in a xenograft mouse model of endometrial cancer (EC).<h4>Methods</h4>Samples were analyzed using tandem mass tags (TMTs) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). DEPs were identified using bioinformatics to determine potential molecular functions and immunohistochemical (IHC) staining.<h4>Results</h4>In total, 11 DEPs were identified in the HRW group relative to the control. The up-regulated proteins included Gatad1, Ttyh3, Nek4, Dyrk2, and Gimap1, while the down-regulated proteins included SP1, Msl1, Plekha7, Dtwd2, MSRA, Translational cancer research1295-1312https://www.ebi.ac.uk/biostudies/studies/S-EPMC8797453biostudies-literatureKRAS, YWHAE, SP1 and MSRA as biomarkers in endometrial cancer.PMC8797453Sang ZYYang YZhu YPMa JWu SFfalseKRAS, YWHAE, SP1 and MSRA as biomarkers in endometrial cancer.<h4>Background</h4>We demonstrated that drinking hydrogen-rich water (HRW) inhibits endometrial tumor growth in our previous work. This research is to identify differentially expressed proteins (DEPs) between HRW and purified water groups in a xenograft mouse model of endometrial cancer (EC).<h4>Methods</h4>Samples were analyzed using tandem mass tags (TMTs) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). DEPs were identified using bioinformatics to determine potential molecular functions and immunohistochemical (IHC) staining.<h4>Results</h4>In total, 11 DEPs were identified in the HRW group relative to the control. The up-regulated proteins included Gatad1, Ttyh3, Nek4, Dyrk2, and Gimap1, while the down-regulated proteins included SP1, Msl1, Plekha7, Dtwd2, MSRA, 2021-01-01T00:00:00Z2021 Mar2025-04-19T09:44:05.681Z2022-02-11T15:36:11.104ZS-EPMC87974533511645610.21037/tcr-20-2969