{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["143"],"submitter":["Yu J"],"pubmed_abstract":["Numerous serological detection kits are being rapidly developed and approved for screening and diagnosing suspected coronavirus disease 2019 (COVID-19) cases. However, cross-reactivity between pre-existing antibodies against other coronaviruses and the captured antigens in these kits can affect detection accuracy, emphasizing the necessity for identifying highly specific antigen fragments for antibody detection. Thus, we performed a conservation and specificity analysis of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid (N) protein. We also integrated various B-cell epitope prediction methods to obtain possible dominant epitope regions for the N protein, analyzed the differences in serological antibody levels for different epitopes using ELISA, and ident"],"journal":["Computers in biology and medicine"],"pagination":["105272"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8799378"],"repository":["biostudies-literature"],"pubmed_title":["High-specificity targets in SARS-CoV-2 N protein for serological detection and distinction from SARS-CoV."],"pmcid":["PMC8799378"],"pubmed_authors":["Yu N","Yao J","Wu Q","Zhao W","Xiao W","Yu J","Zhou X","Liu X","Wan C","Wen K","Qin Z","Zhu L","He X","Zhang B"],"additional_accession":[]},"is_claimable":false,"name":"High-specificity targets in SARS-CoV-2 N protein for serological detection and distinction from SARS-CoV.","description":"Numerous serological detection kits are being rapidly developed and approved for screening and diagnosing suspected coronavirus disease 2019 (COVID-19) cases. However, cross-reactivity between pre-existing antibodies against other coronaviruses and the captured antigens in these kits can affect detection accuracy, emphasizing the necessity for identifying highly specific antigen fragments for antibody detection. Thus, we performed a conservation and specificity analysis of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid (N) protein. We also integrated various B-cell epitope prediction methods to obtain possible dominant epitope regions for the N protein, analyzed the differences in serological antibody levels for different epitopes using ELISA, and ident","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2025-04-19T08:47:05.585Z","creation":"2022-02-11T15:37:34.984Z"},"accession":"S-EPMC8799378","cross_references":{"pubmed":["35121361"],"doi":["10.1016/j.compbiomed.2022.105272"]}}