{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bielajew BJ"],"funding":["NIDCR NIH HHS","NIAMS NIH HHS"],"pagination":["1742S-1754S"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8804780"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(2_suppl)"],"pubmed_abstract":["<h4>Introduction</h4>This study develops assays to quantify collagen subtypes and crosslinks with liquid chromatography-mass spectrometry (LC-MS) and characterizes the cartilages in the Yucatan minipig.<h4>Methods</h4>For collagen subtyping, liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed on tissues digested in trypsin. For collagen crosslinks, LC-MS analysis was performed on hydrolysates. Samples were also examined histologically and with bottom-up proteomics. Ten cartilages (femoral condyle, femoral head, facet joint, floating rib, true rib, auricular cartilage, annulus fibrosus, 2 meniscus locations, and temporomandibular joint disc) were analyzed.<h4>Results</h4>The collagen subtyping assay quantified collagen types I and II. The collagen crosslinks ass"],"journal":["Cartilage"],"pubmed_title":["Methodology to Quantify Collagen Subtypes and Crosslinks: Application in Minipig Cartilages."],"pmcid":["PMC8804780"],"funding_grant_id":["R01 DE015038","R01 AR071457","R01 AR067821"],"pubmed_authors":["Hu JC","Bielajew BJ","Athanasiou KA"],"additional_accession":[]},"is_claimable":false,"name":"Methodology to Quantify Collagen Subtypes and Crosslinks: Application in Minipig Cartilages.","description":"<h4>Introduction</h4>This study develops assays to quantify collagen subtypes and crosslinks with liquid chromatography-mass spectrometry (LC-MS) and characterizes the cartilages in the Yucatan minipig.<h4>Methods</h4>For collagen subtyping, liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed on tissues digested in trypsin. For collagen crosslinks, LC-MS analysis was performed on hydrolysates. Samples were also examined histologically and with bottom-up proteomics. Ten cartilages (femoral condyle, femoral head, facet joint, floating rib, true rib, auricular cartilage, annulus fibrosus, 2 meniscus locations, and temporomandibular joint disc) were analyzed.<h4>Results</h4>The collagen subtyping assay quantified collagen types I and II. The collagen crosslinks ass","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Dec","modification":"2025-04-04T07:51:09.373Z","creation":"2025-04-04T07:51:09.373Z"},"accession":"S-EPMC8804780","cross_references":{"pubmed":["34823380"],"doi":["10.1177/19476035211060508"]}}