biostudies-literatureBielajew BJNIDCR NIH HHSNIAMS NIH HHS1742S-1754Shttps://www.ebi.ac.uk/biostudies/studies/S-EPMC8804780biostudies-literatureUnknown13(2_suppl)<h4>Introduction</h4>This study develops assays to quantify collagen subtypes and crosslinks with liquid chromatography-mass spectrometry (LC-MS) and characterizes the cartilages in the Yucatan minipig.<h4>Methods</h4>For collagen subtyping, liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed on tissues digested in trypsin. For collagen crosslinks, LC-MS analysis was performed on hydrolysates. Samples were also examined histologically and with bottom-up proteomics. Ten cartilages (femoral condyle, femoral head, facet joint, floating rib, true rib, auricular cartilage, annulus fibrosus, 2 meniscus locations, and temporomandibular joint disc) were analyzed.<h4>Results</h4>The collagen subtyping assay quantified collagen types I and II. The collagen crosslinks assCartilageMethodology to Quantify Collagen Subtypes and Crosslinks: Application in Minipig Cartilages.PMC8804780R01 DE015038R01 AR071457R01 AR067821Hu JCBielajew BJAthanasiou KAfalseMethodology to Quantify Collagen Subtypes and Crosslinks: Application in Minipig Cartilages.<h4>Introduction</h4>This study develops assays to quantify collagen subtypes and crosslinks with liquid chromatography-mass spectrometry (LC-MS) and characterizes the cartilages in the Yucatan minipig.<h4>Methods</h4>For collagen subtyping, liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed on tissues digested in trypsin. For collagen crosslinks, LC-MS analysis was performed on hydrolysates. Samples were also examined histologically and with bottom-up proteomics. Ten cartilages (femoral condyle, femoral head, facet joint, floating rib, true rib, auricular cartilage, annulus fibrosus, 2 meniscus locations, and temporomandibular joint disc) were analyzed.<h4>Results</h4>The collagen subtyping assay quantified collagen types I and II. The collagen crosslinks ass2021-01-01T00:00:00Z2021 Dec2025-04-04T07:51:09.373Z2025-04-04T07:51:09.373ZS-EPMC88047803482338010.1177/19476035211060508