biostudies-literatureChen YYShanxi Provincial Key Research and Development ProjectNational Natural Science Foundation of China114632https://www.ebi.ac.uk/biostudies/studies/S-EPMC8806404biostudies-literatureUnknown211The incidence of depression has increased significantly during the COVID-19 pandemic. This disease is closely associated with serotonin _1A (5-HT_1A) receptor and often treated by complex prescription containing Curcuma wenyujin Y. H. Chen et C. Ling. Therefore, we hypothesized that this herb contains bioactive compounds specially binding to the receptor. However, the rapid discovery of new ligands of 5-HT_1A receptor is still challenging due to the lack of efficient screening methods. To address this problem, we developed and characterized a novel approach for the rapid screening of ligands by using immobilized 5-HT_1A receptor as the chromatographic stationary phase. Briefly, haloalkane dehalogenase was fused at the C-terminal of 5-HT_1A Journal of pharmaceutical and biomedical analysisPreparation and characterization of immobilized 5-HT_1A receptor stationary phase for high throughput screening of the receptor-binding ligands from complex systems like Curcuma wenyujin Y. H. Chen et C. Ling extract.PMC8806404220741182020ZDLSF05-0721974107Jin YHZhao XFFan HSChen YYLi QShayiranbieke AZhao XfalsePreparation and characterization of immobilized 5-HT_1A receptor stationary phase for high throughput screening of the receptor-binding ligands from complex systems like Curcuma wenyujin Y. H. Chen et C. Ling extract.The incidence of depression has increased significantly during the COVID-19 pandemic. This disease is closely associated with serotonin _1A (5-HT_1A) receptor and often treated by complex prescription containing Curcuma wenyujin Y. H. Chen et C. Ling. Therefore, we hypothesized that this herb contains bioactive compounds specially binding to the receptor. However, the rapid discovery of new ligands of 5-HT_1A receptor is still challenging due to the lack of efficient screening methods. To address this problem, we developed and characterized a novel approach for the rapid screening of ligands by using immobilized 5-HT_1A receptor as the chromatographic stationary phase. Briefly, haloalkane dehalogenase was fused at the C-terminal of 5-HT_1A 2022-01-01T00:00:00Z2022 Mar2026-05-09T20:23:50.16Z2022-02-11T15:37:36.567ZS-EPMC88064043513167210.1016/j.jpba.2022.114632