{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tong S"],"funding":["Chapman University","National Cancer Institute","NCI NIH HHS","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["1701"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8807785"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(1)"],"pubmed_abstract":["Interferon-gamma (IFN-γ) is shown to stimulate melanoma development and progression. However, the underlying mechanism has not been completely defined. Our study aimed to determine the role of neuronal nitric oxide synthase (nNOS)-mediated signaling in IFN-γ-stimulated melanoma progression and the anti-melanoma effects of novel nNOS inhibitors. Our study shows that IFN-γ markedly induced the expression levels of nNOS in melanoma cells associated with increased intracellular nitric oxide (NO) levels. Co-treatment with novel nNOS inhibitors effectively alleviated IFN-γ-activated STAT1/3. Further, reverse phase protein array (RPPA) analysis demonstrated that IFN-γ induced the expression of HIF1α, c-Myc, and programmed death-ligand 1 (PD-L1), in contrast to IFN-α. Blocking the nNOS-mediated si"],"journal":["Scientific reports"],"pubmed_title":["Inhibition of interferon-gamma-stimulated melanoma progression by targeting neuronal nitric oxide synthase (nNOS)."],"pmcid":["PMC8807785"],"funding_grant_id":["R01 GM049725","Faculty Opportunity Grant","R35 GM131788","K08CA179084","K08 CA179084","1R35GM131788-01"],"pubmed_authors":["Silverman RB","Tong S","Patel A","Cinelli MA","Huang H","El-Sayed NS","Yang S"],"additional_accession":[]},"is_claimable":false,"name":"Inhibition of interferon-gamma-stimulated melanoma progression by targeting neuronal nitric oxide synthase (nNOS).","description":"Interferon-gamma (IFN-γ) is shown to stimulate melanoma development and progression. However, the underlying mechanism has not been completely defined. Our study aimed to determine the role of neuronal nitric oxide synthase (nNOS)-mediated signaling in IFN-γ-stimulated melanoma progression and the anti-melanoma effects of novel nNOS inhibitors. Our study shows that IFN-γ markedly induced the expression levels of nNOS in melanoma cells associated with increased intracellular nitric oxide (NO) levels. Co-treatment with novel nNOS inhibitors effectively alleviated IFN-γ-activated STAT1/3. Further, reverse phase protein array (RPPA) analysis demonstrated that IFN-γ induced the expression of HIF1α, c-Myc, and programmed death-ligand 1 (PD-L1), in contrast to IFN-α. Blocking the nNOS-mediated si","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Feb","modification":"2025-04-05T13:57:53.367Z","creation":"2025-04-05T13:57:53.367Z"},"accession":"S-EPMC8807785","cross_references":{"pubmed":["35105915"],"doi":["10.1038/s41598-022-05394-6"]}}