{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nagaishi T"],"funding":["Scientific Research-C from the Japanese Ministry of Education, Culture, Sports, Science and Technology","Joint Usage/Research Program of TMDU Medical Research Institute","Challenging Exploratory Research from the Japanese Ministry of Education, Culture, Sports, Science and Technology","RO1 Grants from the National Institutes of Health","Tojuro Iijima Foundation for Food Science and Technology","Nipponham Foundation","Japan Foundation for Applied Enzymology","Foundation for Advancement of International Science","Scientific Research on Innovative Area from the Japanese Ministry of Education, Culture, Sports, Science and Technology","Practical Research Project for Rare/Intractable Disease from Japan Agency for Medical Research and Development","Abbott Japan Allergy Research Award","Scientific Research-S from the Japanese Ministry of Education, Culture, Sports, Science and Technology","NIDDK NIH HHS","Scientific Research-B from the Japanese Ministry of Education, Culture, Sports, Science and Technology","Takeda Science Foundation","Mishima Kaiun Memorial Foundation","Memorial Fund of Nihon University Medical Alumni Association","Yamada Foundation","Naoki Tsuchida Memorial Research Grant"],"pagination":["487-496"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8809603"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["71(3)"],"pubmed_abstract":["<h4>Objective</h4>Although immunoglobulin A (IgA) is abundantly expressed in the gut and known to be an important component of mucosal barriers against luminal pathogens, its precise function remains unclear. Therefore, we tried to elucidate the effect of IgA on gut homeostasis maintenance and its mechanism.<h4>Design</h4>We generated various IgA mutant mouse lines using the CRISPR/Cas9 genome editing system. Then, we evaluated the effect on the small intestinal homeostasis, pathology, intestinal microbiota, cytokine production, and immune cell activation using intravital imaging.<h4>Results</h4>We obtained two lines, with one that contained a <50 base pair deletion in the cytoplasmic region of the IgA allele (IgA tail-mutant; IgA<sup>tm/tm</sup>) and the other that lacked the most constan"],"journal":["Gut"],"pubmed_title":["Immunoglobulin A-specific deficiency induces spontaneous inflammation specifically in the ileum."],"pmcid":["PMC8809603"],"funding_grant_id":["R37 DK044319","DK088199, DK44319 and DK51362","15K08526 and 18K07997","R01 DK088199","R01 DK051362","16eK0109047h0003","26221307","R01 DK044319","22117508","15K15288","16H05286 and 20H03658"],"pubmed_authors":["Kumazawa T","Ishino F","Usami T","Blumberg RS","Ono R","Miura T","Tsugawa N","Kotake K","Karasuyama H","Kawasaki H","Yoshikawa S","Yamada D","Watabe T","Hirayama K","Aida T","Watanabe M","Hirakata S","Nagaishi T","Tanaka K","Okamoto R","Adachi T"],"additional_accession":[]},"is_claimable":false,"name":"Immunoglobulin A-specific deficiency induces spontaneous inflammation specifically in the ileum.","description":"<h4>Objective</h4>Although immunoglobulin A (IgA) is abundantly expressed in the gut and known to be an important component of mucosal barriers against luminal pathogens, its precise function remains unclear. Therefore, we tried to elucidate the effect of IgA on gut homeostasis maintenance and its mechanism.<h4>Design</h4>We generated various IgA mutant mouse lines using the CRISPR/Cas9 genome editing system. Then, we evaluated the effect on the small intestinal homeostasis, pathology, intestinal microbiota, cytokine production, and immune cell activation using intravital imaging.<h4>Results</h4>We obtained two lines, with one that contained a <50 base pair deletion in the cytoplasmic region of the IgA allele (IgA tail-mutant; IgA<sup>tm/tm</sup>) and the other that lacked the most constan","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2026-05-03T22:39:42.308Z","creation":"2025-04-19T13:19:30.048Z"},"accession":"S-EPMC8809603","cross_references":{"pubmed":["33963042"],"doi":["10.1136/gutjnl-2020-322873"]}}