{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Guo Z"],"funding":["the Anhui Provincial Education Department Fund","Anhui Provincial Medical and Health Care Key Specialties Construction Fund, and the University Fund of Anhui Medical University"],"pagination":["9869-9884"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8810030"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(2)"],"pubmed_abstract":["DNA damage plays a key role in various biological processes involved in malignant disease, the role of the DNA damage repair gene <i>EME1</i> (essential meiotic structure-specific endonuclease 1) in gastric cancer (GC) development is unknown. This work aimed to investigate expression and role of <i>EME1</i> in tumorigenesis. Quantitative real-time polymerase chain reaction (qRT-PCR), immunoblot, cell viability and dual-luciferase reporter assays, RNAi and gene transfection, and immunofluorescent staining were performed to assess <i>EME1</i> regulation in GC tumorigenesis. Further, mouse xenografts were established for <i>in vivo</i> mechanistic studies. <i>EME1</i> was found to be upregulated in both gastric cancer cells and clinically obtained tumors. Additionally, <i>EME1</i> levels were"],"journal":["Bioengineered"],"pubmed_title":["Essential meiotic structure-specific endonuclease1 (<i>EME1</i>) promotes malignant features in gastric cancer cells via the Akt/GSK3B/CCND1 pathway."],"pmcid":["PMC8810030"],"funding_grant_id":["2019A1","2019xkj242","2020jyxm2058"],"pubmed_authors":["Li W","Liang E","Jiang L","Guo Z","Zhi F"],"additional_accession":[]},"is_claimable":false,"name":"Essential meiotic structure-specific endonuclease1 (<i>EME1</i>) promotes malignant features in gastric cancer cells via the Akt/GSK3B/CCND1 pathway.","description":"DNA damage plays a key role in various biological processes involved in malignant disease, the role of the DNA damage repair gene <i>EME1</i> (essential meiotic structure-specific endonuclease 1) in gastric cancer (GC) development is unknown. This work aimed to investigate expression and role of <i>EME1</i> in tumorigenesis. Quantitative real-time polymerase chain reaction (qRT-PCR), immunoblot, cell viability and dual-luciferase reporter assays, RNAi and gene transfection, and immunofluorescent staining were performed to assess <i>EME1</i> regulation in GC tumorigenesis. Further, mouse xenografts were established for <i>in vivo</i> mechanistic studies. <i>EME1</i> was found to be upregulated in both gastric cancer cells and clinically obtained tumors. Additionally, <i>EME1</i> levels were","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Dec","modification":"2025-04-04T14:59:28.298Z","creation":"2025-04-04T14:59:28.298Z"},"accession":"S-EPMC8810030","cross_references":{"pubmed":["34719326"],"doi":["10.1080/21655979.2021.1999371"]}}