{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13(6)"],"submitter":["Chehreara R"],"pubmed_abstract":["<h4>Background and objectives</h4>Epsilon toxin is the third hazardous bacterial toxin causing ABS enterotoxaemia in domestic animal. In addition, epsilon toxin is known as a biological warfare agent. The aim of this study was to produce the recombinant mature epsilon toxin to evaluate cell death impact on the kidney cell line.<h4>Materials and methods</h4>For this purpose, the sequence of mature epsilon toxin (46-328 aa) in pET28a was cloned and expressed in <i>Escherichia coli</i> BL21 (DE3) and purified by nickel-nitrilotriacetic acid (Ni-NTA) column and confirmed by western blot analysis using HRP conjugated anti-His antibody. Then, to assess the anti-proliferative effects of different concentrations of recombinant epsilon toxin, the MTT assay was done on the HEK293 cell line. The anne"],"journal":["Iranian journal of microbiology"],"pagination":["832-838"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8816699"],"repository":["biostudies-literature"],"pubmed_title":["Cell death analysis of recombinant mature epsilon toxin on the kidney cell line."],"pmcid":["PMC8816699"],"pubmed_authors":["Hosseini HM","Chehreara R","Karizi SZ","Amani J","Mirhosseini SA","Kazemi R","Shafiei M"],"additional_accession":[]},"is_claimable":false,"name":"Cell death analysis of recombinant mature epsilon toxin on the kidney cell line.","description":"<h4>Background and objectives</h4>Epsilon toxin is the third hazardous bacterial toxin causing ABS enterotoxaemia in domestic animal. In addition, epsilon toxin is known as a biological warfare agent. The aim of this study was to produce the recombinant mature epsilon toxin to evaluate cell death impact on the kidney cell line.<h4>Materials and methods</h4>For this purpose, the sequence of mature epsilon toxin (46-328 aa) in pET28a was cloned and expressed in <i>Escherichia coli</i> BL21 (DE3) and purified by nickel-nitrilotriacetic acid (Ni-NTA) column and confirmed by western blot analysis using HRP conjugated anti-His antibody. Then, to assess the anti-proliferative effects of different concentrations of recombinant epsilon toxin, the MTT assay was done on the HEK293 cell line. The anne","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Dec","modification":"2025-04-04T07:55:05.739Z","creation":"2025-04-04T07:55:05.739Z"},"accession":"S-EPMC8816699","cross_references":{"pubmed":["35222862"],"doi":["10.18502/ijm.v13i6.8088"]}}