biostudies-literatureUnknown13(6)Chehreara R<h4>Background and objectives</h4>Epsilon toxin is the third hazardous bacterial toxin causing ABS enterotoxaemia in domestic animal. In addition, epsilon toxin is known as a biological warfare agent. The aim of this study was to produce the recombinant mature epsilon toxin to evaluate cell death impact on the kidney cell line.<h4>Materials and methods</h4>For this purpose, the sequence of mature epsilon toxin (46-328 aa) in pET28a was cloned and expressed in <i>Escherichia coli</i> BL21 (DE3) and purified by nickel-nitrilotriacetic acid (Ni-NTA) column and confirmed by western blot analysis using HRP conjugated anti-His antibody. Then, to assess the anti-proliferative effects of different concentrations of recombinant epsilon toxin, the MTT assay was done on the HEK293 cell line. The anneIranian journal of microbiology832-838https://www.ebi.ac.uk/biostudies/studies/S-EPMC8816699biostudies-literatureCell death analysis of recombinant mature epsilon toxin on the kidney cell line.PMC8816699Hosseini HMChehreara RKarizi SZAmani JMirhosseini SAKazemi RShafiei MfalseCell death analysis of recombinant mature epsilon toxin on the kidney cell line.<h4>Background and objectives</h4>Epsilon toxin is the third hazardous bacterial toxin causing ABS enterotoxaemia in domestic animal. In addition, epsilon toxin is known as a biological warfare agent. The aim of this study was to produce the recombinant mature epsilon toxin to evaluate cell death impact on the kidney cell line.<h4>Materials and methods</h4>For this purpose, the sequence of mature epsilon toxin (46-328 aa) in pET28a was cloned and expressed in <i>Escherichia coli</i> BL21 (DE3) and purified by nickel-nitrilotriacetic acid (Ni-NTA) column and confirmed by western blot analysis using HRP conjugated anti-His antibody. Then, to assess the anti-proliferative effects of different concentrations of recombinant epsilon toxin, the MTT assay was done on the HEK293 cell line. The anne2021-01-01T00:00:00Z2021 Dec2025-04-04T07:55:05.739Z2025-04-04T07:55:05.739ZS-EPMC88166993522286210.18502/ijm.v13i6.8088