biostudies-literatureUnknown12Deng APSulfur Angelicae Dahuricae Radix (Baizhi) is a common medicinal herb in Asian countries. A practical protocol combining metabolomics, pharmacology, and cytotoxicity was developed to comprehensively evaluate the influence of sulfur-fumigation on the quality of Baizhi. Furocoumarins could be transformed into sulfur-containing compounds during the sulfuring process, among which <b>1</b> and <b>3</b> were purified with relatively high abundance and identified as 3,4-dihydrobyakangelicin-4-sulfonic acid and (4<i>R</i>,12<i>S</i>)-3,4-dihydrooxypeucedanin hydrate-4-sulfonic acid (OXH-S), respectively. OXH-S was found to be an addition product of sulfite and oxypeucedanin hydrate (OXH-N). Then, the cytotoxicity and anti-inflammatory activity of OXH-N, OXH-S, and water extracts of sulfured (extracFrontiers in pharmacology799504https://www.ebi.ac.uk/biostudies/studies/S-EPMC8822044biostudies-literaturePractical Protocol for Comprehensively Evaluating Sulfur-Fumigation of Baizhi Based on Metabolomics, Pharmacology, and Cytotoxicity.PMC8822044Zhou LZhang WJKang LPLyu CGZhan ZLWang SGuo LPHuang LQDeng APNan TGKang CZWang HYfalsePractical Protocol for Comprehensively Evaluating Sulfur-Fumigation of Baizhi Based on Metabolomics, Pharmacology, and Cytotoxicity.Sulfur Angelicae Dahuricae Radix (Baizhi) is a common medicinal herb in Asian countries. A practical protocol combining metabolomics, pharmacology, and cytotoxicity was developed to comprehensively evaluate the influence of sulfur-fumigation on the quality of Baizhi. Furocoumarins could be transformed into sulfur-containing compounds during the sulfuring process, among which <b>1</b> and <b>3</b> were purified with relatively high abundance and identified as 3,4-dihydrobyakangelicin-4-sulfonic acid and (4<i>R</i>,12<i>S</i>)-3,4-dihydrooxypeucedanin hydrate-4-sulfonic acid (OXH-S), respectively. OXH-S was found to be an addition product of sulfite and oxypeucedanin hydrate (OXH-N). Then, the cytotoxicity and anti-inflammatory activity of OXH-N, OXH-S, and water extracts of sulfured (extrac2021-01-01T00:00:00Z20212025-04-26T03:50:18.252Z2025-04-06T10:58:20.187ZS-EPMC88220443514540410.3389/fphar.2021.799504