{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li J"],"funding":["Natural Science Foundation of Guangxi Province","National Natural Science Foundation of China"],"pagination":["792023"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8823705"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9"],"pubmed_abstract":["2,3,5,6-Tetramethylpyrazine (TMP) is an active pharmaceutical ingredient originally isolated from <i>Ligusticum wallichii</i> for curing cardiovascular and cerebrovascular diseases and is widely used as a popular flavoring additive in the food industry. Hence, there is a great interest in developing new strategies to produce this high-value compound in an ecological and economical way. Herein, a cost-competitive combinational approach was proposed to accomplish green and high-efficiency production of TMP. First, microbial cell factories were constructed to produce acetoin (3-hydroxy-2-butanone, AC), an endogenous precursor of TMP, by introducing a biosynthesis pathway coupled with an intracellular NAD<sup>+</sup> regeneration system to the wild-type <i>Escherichia coli</i>. To further impr"],"journal":["Frontiers in bioengineering and biotechnology"],"pubmed_title":["A Green Route for High-Yield Production of Tetramethylpyrazine From Non-Food Raw Materials."],"pmcid":["PMC8823705"],"funding_grant_id":["2018GXNSFBA281017","21868007 21466007"],"pubmed_authors":["Li J","Lu J","Ma Z","Chen X","Xie N","Diao M"],"additional_accession":[]},"is_claimable":false,"name":"A Green Route for High-Yield Production of Tetramethylpyrazine From Non-Food Raw Materials.","description":"2,3,5,6-Tetramethylpyrazine (TMP) is an active pharmaceutical ingredient originally isolated from <i>Ligusticum wallichii</i> for curing cardiovascular and cerebrovascular diseases and is widely used as a popular flavoring additive in the food industry. Hence, there is a great interest in developing new strategies to produce this high-value compound in an ecological and economical way. Herein, a cost-competitive combinational approach was proposed to accomplish green and high-efficiency production of TMP. First, microbial cell factories were constructed to produce acetoin (3-hydroxy-2-butanone, AC), an endogenous precursor of TMP, by introducing a biosynthesis pathway coupled with an intracellular NAD<sup>+</sup> regeneration system to the wild-type <i>Escherichia coli</i>. To further impr","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2025-04-19T10:47:07.769Z","creation":"2025-04-19T10:47:07.769Z"},"accession":"S-EPMC8823705","cross_references":{"pubmed":["35145961"],"doi":["10.3389/fbioe.2021.792023"]}}