{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["17(2)"],"submitter":["Ripmeester EGJ"],"pubmed_abstract":["BMP7 is a morphogen capable of counteracting the OA chondrocyte hypertrophic phenotype via NKX3-2. NKX3-2 represses expression of RUNX2, an important transcription factor for chondrocyte hypertrophy. Since RUNX2 has previously been described as an inhibitor for 47S pre-rRNA transcription, we hypothesized that BMP7 positively influences 47S pre-rRNA transcription through NKX3-2, resulting in increased protein translational capacity. Therefor SW1353 cells and human primary chondrocytes were exposed to BMP7 and rRNA (18S, 5.8S, 28S) expression was determined by RT-qPCR. NKX3-2 knockdown was achieved via transfection of a NKX3-2-specific siRNA duplex. Translational capacity was assessed by the SUNsET assay, and 47S pre-rRNA transcription was determined by transfection of a 47S gene promoter-re"],"journal":["PloS one"],"pagination":["e0263430"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8827423"],"repository":["biostudies-literature"],"pubmed_title":["BMP7 increases protein synthesis in SW1353 cells and determines rRNA levels in a NKX3-2-dependent manner."],"pmcid":["PMC8827423"],"pubmed_authors":["Surtel DAM","van Rhijn LW","van den Akker GGH","Caron MMJ","Ripmeester EGJ","Cremers A","Welting TJM","Steijns JSJ"],"additional_accession":[]},"is_claimable":false,"name":"BMP7 increases protein synthesis in SW1353 cells and determines rRNA levels in a NKX3-2-dependent manner.","description":"BMP7 is a morphogen capable of counteracting the OA chondrocyte hypertrophic phenotype via NKX3-2. NKX3-2 represses expression of RUNX2, an important transcription factor for chondrocyte hypertrophy. Since RUNX2 has previously been described as an inhibitor for 47S pre-rRNA transcription, we hypothesized that BMP7 positively influences 47S pre-rRNA transcription through NKX3-2, resulting in increased protein translational capacity. Therefor SW1353 cells and human primary chondrocytes were exposed to BMP7 and rRNA (18S, 5.8S, 28S) expression was determined by RT-qPCR. NKX3-2 knockdown was achieved via transfection of a NKX3-2-specific siRNA duplex. Translational capacity was assessed by the SUNsET assay, and 47S pre-rRNA transcription was determined by transfection of a 47S gene promoter-re","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-22T04:47:01.927Z","creation":"2025-04-05T21:03:27.782Z"},"accession":"S-EPMC8827423","cross_references":{"pubmed":["35139106"],"doi":["10.1371/journal.pone.0263430"]}}