{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cai GP"],"funding":["Hunan Provincial Science and Technology Department","the innovation-driven project of Central South University","National Natural Science Foundation of China"],"pagination":["e13178"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8828262"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["55(2)"],"pubmed_abstract":["<h4>Objectives</h4>DNA N6-methyladenine (N6-mA) demethylase Alkbh1 participates in regulating osteogenic differentiation of mesenchymal stem cell (MSCs) and vascular calcification. However, the role of Alkbh1 in bone metabolism remains unclear.<h4>Materials and methods</h4>Bone marrow mesenchymal stem cells (BMSCs)-specific Alkbh1 knockout mice were used to investigate the role of Alkbh1 in bone metabolism. Western blot, qRT-PCR, and immunofluorescent staining were used to evaluate the expression of Alkbh1 or optineurin (optn). Micro-CT, histomorphometric analysis, and calcein double-labeling assay were used to evaluate bone phenotypes. Cell staining and qRT-PCR were used to evaluate the osteogenic or adipogenic differentiation of BMSCs. Dot blotting was used to detect the level of N6-mA i"],"journal":["Cell proliferation"],"pubmed_title":["Alkbh1-mediated DNA N6-methyladenine modification regulates bone marrow mesenchymal stem cell fate during skeletal aging."],"pmcid":["PMC8828262"],"funding_grant_id":["81900810","81770877","20180033040008","2020RC2011","82000811","82170902"],"pubmed_authors":["Wang M","Jiang TJ","Cai GP","Liu YL","Xiao Y","Luo LP","Yuan J"],"additional_accession":[]},"is_claimable":false,"name":"Alkbh1-mediated DNA N6-methyladenine modification regulates bone marrow mesenchymal stem cell fate during skeletal aging.","description":"<h4>Objectives</h4>DNA N6-methyladenine (N6-mA) demethylase Alkbh1 participates in regulating osteogenic differentiation of mesenchymal stem cell (MSCs) and vascular calcification. However, the role of Alkbh1 in bone metabolism remains unclear.<h4>Materials and methods</h4>Bone marrow mesenchymal stem cells (BMSCs)-specific Alkbh1 knockout mice were used to investigate the role of Alkbh1 in bone metabolism. Western blot, qRT-PCR, and immunofluorescent staining were used to evaluate the expression of Alkbh1 or optineurin (optn). Micro-CT, histomorphometric analysis, and calcein double-labeling assay were used to evaluate bone phenotypes. Cell staining and qRT-PCR were used to evaluate the osteogenic or adipogenic differentiation of BMSCs. Dot blotting was used to detect the level of N6-mA i","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Feb","modification":"2025-04-25T17:37:21.49Z","creation":"2025-04-25T17:37:21.49Z"},"accession":"S-EPMC8828262","cross_references":{"pubmed":["35018683"],"doi":["10.1111/cpr.13178"]}}