biostudies-literatureUnknown13Dobano CCOVID-19 affects children to a lesser extent than adults but they can still get infected and transmit SARS-CoV-2 to their contacts. Field deployable non-invasive sensitive diagnostic techniques are needed to evaluate the infectivity dynamics of SARS-CoV-2 in pediatric populations and guide public health interventions, particularly if this population is not fully vaccinated. We evaluated the utility of high-throughput Luminex assays to quantify saliva IgM, IgA and IgG antibodies against five SARS-CoV-2 spike (S) and nucleocapsid (N) antigens in a contacts and infectivity longitudinal study in 122 individuals (52 children and 70 adults). We compared saliva versus serum/plasma samples in infected children and adults diagnosed by weekly RT-PCR over 35 days (n=62), and those who consistently teFrontiers in immunology751705https://www.ebi.ac.uk/biostudies/studies/S-EPMC8828491biostudies-literatureMultiplex Antibody Analysis of IgM, IgA and IgG to SARS-CoV-2 in Saliva and Serum From Infected Children and Their Close Contacts.PMC8828491Gratacos EFernandez de Sevilla MAlonso SMoncunill GGarcia-Miquel ACodina ARodrigo Melero NRubio RPuyol LBalanza NAguilar RSantano RPons Tomas GJordan ICarolis CBaro BFumado VCubells MGarcia-Garcia JJDobano CBassat QIzquierdo LFortuny CClaverol JJimenez AGirona-Alarcon MArias SMunoz-Almagro CMillat-Martinez PMele Casas MBarrios DHernandez Garcia MBonet-Carne EVidal MAjanovic SfalseMultiplex Antibody Analysis of IgM, IgA and IgG to SARS-CoV-2 in Saliva and Serum From Infected Children and Their Close Contacts.COVID-19 affects children to a lesser extent than adults but they can still get infected and transmit SARS-CoV-2 to their contacts. Field deployable non-invasive sensitive diagnostic techniques are needed to evaluate the infectivity dynamics of SARS-CoV-2 in pediatric populations and guide public health interventions, particularly if this population is not fully vaccinated. We evaluated the utility of high-throughput Luminex assays to quantify saliva IgM, IgA and IgG antibodies against five SARS-CoV-2 spike (S) and nucleocapsid (N) antigens in a contacts and infectivity longitudinal study in 122 individuals (52 children and 70 adults). We compared saliva versus serum/plasma samples in infected children and adults diagnosed by weekly RT-PCR over 35 days (n=62), and those who consistently te2022-01-01T00:00:00Z20222025-04-26T02:01:42.38Z2025-02-19T01:34:24.872ZS-EPMC88284913515409410.3389/fimmu.2022.751705