biostudies-literatureQi LChina Postdoctoral Science FoundationNational Natural Science Foundation of China1264-1280https://www.ebi.ac.uk/biostudies/studies/S-EPMC8831944biostudies-literatureUnknown26(4)Ventricular arrhythmias (VAs) triggers by sympathetic nerve hyperactivity contribute to sudden cardiac death in myocardial infarction (MI) patients. Microglia-mediated inflammation in the paraventricular nucleus (PVN) is involved in sympathetic hyperactivity after MI. N6-methyladenosine (m⁶ A), the most prevalent mRNA and epigenetic modification, is critical for mediating cell inflammation. We aimed to explore whether METTL3-mediated m⁶ A modification is involved in microglia-mediated sympathetic hyperactivity after MI in the PVN. MI model was established by left coronary artery ligation. METTL3-mediated m⁶ A modification was markedly increased in the PVN at 3 days after MI, and METTL3 was primarily located in microglia by immunofluorescence. RNA-seq, MeRIPJournal of cellular and molecular medicineNew insights into the central sympathetic hyperactivity post-myocardial infarction: Roles of METTL3-mediated m⁶ A methylation.PMC88319442016M60215481870253882070345Qi LLi PYan SWang KZhao YShi YLi XLi YFeng MHu HLyu HXue MWang YYin JfalseNew insights into the central sympathetic hyperactivity post-myocardial infarction: Roles of METTL3-mediated m⁶ A methylation.Ventricular arrhythmias (VAs) triggers by sympathetic nerve hyperactivity contribute to sudden cardiac death in myocardial infarction (MI) patients. Microglia-mediated inflammation in the paraventricular nucleus (PVN) is involved in sympathetic hyperactivity after MI. N6-methyladenosine (m⁶ A), the most prevalent mRNA and epigenetic modification, is critical for mediating cell inflammation. We aimed to explore whether METTL3-mediated m⁶ A modification is involved in microglia-mediated sympathetic hyperactivity after MI in the PVN. MI model was established by left coronary artery ligation. METTL3-mediated m⁶ A modification was markedly increased in the PVN at 3 days after MI, and METTL3 was primarily located in microglia by immunofluorescence. RNA-seq, MeRIP2022-01-01T00:00:00Z2022 Feb2025-04-04T20:23:56.575Z2025-04-04T20:23:56.575ZS-EPMC88319443504025310.1111/jcmm.17183