biostudies-literatureWu HSelf-Planned Task of State Key Laboratory of Robotics and System418https://www.ebi.ac.uk/biostudies/studies/S-EPMC8840109biostudies-literatureUnknown12(3)Adsorption of urea from dialysate is essential for wearable artificial kidneys (WRK). Molecularly imprinted microspheres with nanoporous and multilayered structures are prepared based on liquid-liquid phase separation (LLPS), which can selectively adsorb urea. In addition, we combine the microspheres with a designed polydimethylsiloxane (PDMS) chip to propose an efficient urea adsorption platform. In this work, we propose a formulation of LLPS including Tripropylene glycol diacrylate (TPGDA), ethanol, and acrylic acid (30% v/v), to prepare urea molecularly imprinted microspheres in a simple and highly controllable method. These microspheres have urea molecular imprinting sites on the surface and inside, allowing selective adsorption of urea and preservation of other essential constituents.Nanomaterials (Basel, Switzerland)Controllable Fabrication of Molecularly Imprinted Microspheres with Nanoporous and Multilayered Structure for Dialysate Regeneration.PMC8840109SKLRS201803BLiu LXue CRen YJiang HChen XZhang SWu WWu HfalseControllable Fabrication of Molecularly Imprinted Microspheres with Nanoporous and Multilayered Structure for Dialysate Regeneration.Adsorption of urea from dialysate is essential for wearable artificial kidneys (WRK). Molecularly imprinted microspheres with nanoporous and multilayered structures are prepared based on liquid-liquid phase separation (LLPS), which can selectively adsorb urea. In addition, we combine the microspheres with a designed polydimethylsiloxane (PDMS) chip to propose an efficient urea adsorption platform. In this work, we propose a formulation of LLPS including Tripropylene glycol diacrylate (TPGDA), ethanol, and acrylic acid (30% v/v), to prepare urea molecularly imprinted microspheres in a simple and highly controllable method. These microspheres have urea molecular imprinting sites on the surface and inside, allowing selective adsorption of urea and preservation of other essential constituents.2022-01-01T00:00:00Z2022 Jan2025-04-26T03:57:23.935Z2025-04-06T10:58:02.197ZS-EPMC88401093515976610.3390/nano12030418