{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Klykov O"],"funding":["Simons Foundation","NCRR NIH HHS","EPIC-XS","Dutch Research Council (NWO)","Horizon 2020","National Institutes of Health","NIGMS NIH HHS"],"pagination":["285-303"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8842845"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["82(2)"],"pubmed_abstract":["Combining diverse experimental structural and interactomic methods allows for the construction of comprehensible molecular encyclopedias of biological systems. Typically, this involves merging several independent approaches that provide complementary structural and functional information from multiple perspectives and at different resolution ranges. A particularly potent combination lies in coupling structural information from cryoelectron microscopy or tomography (cryo-EM or cryo-ET) with interactomic and structural information from mass spectrometry (MS)-based structural proteomics. Cryo-EM/ET allows for sub-nanometer visualization of biological specimens in purified and near-native states, while MS provides bioanalytical information for proteins and protein complexes without introducing"],"journal":["Molecular cell"],"pubmed_title":["Label-free visual proteomics: Coupling MS- and EM-based approaches in structural biology."],"pmcid":["PMC8842845"],"funding_grant_id":["741.018.201","SF349247","P41 GM103310","U24GM139171","GM103310","U24 GM129539","U24GM129539","U24 GM139171","P41 RR001209","823839"],"pubmed_authors":["Klykov O","Heck AJR","Carragher B","Noble AJ","Kopylov M","Scheltema RA"],"additional_accession":[]},"is_claimable":false,"name":"Label-free visual proteomics: Coupling MS- and EM-based approaches in structural biology.","description":"Combining diverse experimental structural and interactomic methods allows for the construction of comprehensible molecular encyclopedias of biological systems. Typically, this involves merging several independent approaches that provide complementary structural and functional information from multiple perspectives and at different resolution ranges. A particularly potent combination lies in coupling structural information from cryoelectron microscopy or tomography (cryo-EM or cryo-ET) with interactomic and structural information from mass spectrometry (MS)-based structural proteomics. Cryo-EM/ET allows for sub-nanometer visualization of biological specimens in purified and near-native states, while MS provides bioanalytical information for proteins and protein complexes without introducing","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2025-04-04T08:33:35.649Z","creation":"2025-04-04T08:33:35.649Z"},"accession":"S-EPMC8842845","cross_references":{"pubmed":["35063097"],"doi":["10.1016/j.molcel.2021.12.027"]}}