biostudies-literatureTong HYNational Major New Drugs Innovation and Development5023654https://www.ebi.ac.uk/biostudies/studies/S-EPMC8843775biostudies-literatureUnknown2022<h4>Background</h4>Anshen Buxin Liuwei pill (ABLP) is a Mongolian medicinal formula which has a therapeutic effect on the symptoms such as coronary heart disease, angina pectoris, arrhythmia, depression and irritability, palpitation, and short breath. However, its bioactivity against cardiac injury remains unclear.<h4>Methods</h4>The protective effect of ABLP was evaluated using H9c2 cells. Cell viability, intracellular Ca²⁺, reactive oxidative indices, and mitochondrial membrane potential (∆ψ) were assessed, respectively. The mRNA levels of Ca²⁺ channel-related genes (DHPR, RyR2, and SCN5A) and oxidative stress-related genes (Keap1, Nrf2, and HO-1) were measured by RT-PCR.<h4>Results</h4>0.5-50 <i>μ</i>g/mL ABLP could significantly decrease H₂O₂-induced cell injury by suppressing cell necrosis/apoptosis and excess oxidative stress, ameliorating the collapse of ∆ψ, and reducing intracellular Ca²⁺ concentration. Furthermore, 0.5-50 <i>μ</i>g/mL ABLP reversed H₂O₂-induced imbalance in the mRNA levels of DHPR, RyR2, SCN5A, Keap1, Nrf2, and HO-1 gene in H9c2 cells, which further illustrate the mechanism.<h4>Conclusion</h4>ABLP provided protective and therapeutic benefits against H₂O₂-induced H9c2 cell injury, indicating that this formula can effectively treat coronary disease. In addition, the present study also provides an in-depth understanding of the pharmacological functions of ABLP, which may lead to further successful applications of Mongolian medicine.Evidence-based complementary and alternative medicine : eCAMAnshen Buxin Liuwei Pill, a Mongolian Medicinal Formula, Could Protect H₂O₂-Induced H9c2 Myocardial Cell Injury by Suppressing Apoptosis, Calcium Channel Activation, and Oxidative Stress.PMC88437752018YFC1708206Murtaza GDong YHuang XJHuang YJTong HYSarfaraz Iqbal MfalseAnshen Buxin Liuwei Pill, a Mongolian Medicinal Formula, Could Protect H₂O₂-Induced H9c2 Myocardial Cell Injury by Suppressing Apoptosis, Calcium Channel Activation, and Oxidative Stress.<h4>Background</h4>Anshen Buxin Liuwei pill (ABLP) is a Mongolian medicinal formula which has a therapeutic effect on the symptoms such as coronary heart disease, angina pectoris, arrhythmia, depression and irritability, palpitation, and short breath. However, its bioactivity against cardiac injury remains unclear.<h4>Methods</h4>The protective effect of ABLP was evaluated using H9c2 cells. Cell viability, intracellular Ca²⁺, reactive oxidative indices, and mitochondrial membrane potential (∆ψ) were assessed, respectively. The mRNA levels of Ca²⁺ channel-related genes (DHPR, RyR2, and SCN5A) and oxidative stress-related genes (Keap1, Nrf2, and HO-1) were measured by RT-PCR.<h4>Results</h4>0.5-50 <i>μ</i>g/mL ABLP could significantly decrease H₂O₂-induced cell injury by suppressing cell necrosis/apoptosis and excess oxidative stress, ameliorating the collapse of ∆ψ, and reducing intracellular Ca²⁺ concentration. Furthermore, 0.5-50 <i>μ</i>g/mL ABLP reversed H₂O₂-induced imbalance in the mRNA levels of DHPR, RyR2, SCN5A, Keap1, Nrf2, and HO-1 gene in H9c2 cells, which further illustrate the mechanism.<h4>Conclusion</h4>ABLP provided protective and therapeutic benefits against H₂O₂-induced H9c2 cell injury, indicating that this formula can effectively treat coronary disease. In addition, the present study also provides an in-depth understanding of the pharmacological functions of ABLP, which may lead to further successful applications of Mongolian medicine.2022-01-01T00:00:00Z20222024-11-14T20:01:00.702Z2024-11-14T20:01:00.702ZS-EPMC88437753517810410.1155/2022/5023654