biostudies-literatureUnknown13Pankaew SCanceropôle PACAAgence Nationale de la RechercheHorizon 2020 Framework ProgrammeCentre National de la Recherche ScientifiqueInstitut National de la Santé et de la Recherche MédicalePTEN (Phosphatase and TENsin homolog) is a well-known tumor suppressor involved in numerous types of cancer, including T-cell acute lymphoblastic leukemia (T-ALL). In human, loss-of-function mutations of <i>PTEN</i> are correlated to mature T-ALL expressing a T-cell receptor (TCR) at their cell surface. In accordance with human T-ALL, inactivation of <i>Pten</i> gene in mouse thymocytes induces TCRαβ⁺ T-ALL development. Herein, we explored the functional interaction between TCRαβ signaling and PTEN. First, we performed single-cell RNA sequencing (scRNAseq) of PTEN-deficient and PTEN-proficient thymocytes. Bioinformatic analysis of our scRNAseq data showed that pathological Pten^del thymocytes express, as expected, <i>Myc</i> transcript, whereas inference of pathway actFrontiers in immunology797244https://www.ebi.ac.uk/biostudies/studies/S-EPMC8847596biostudies-literatureCalcium Signaling Is Impaired in PTEN-Deficient T Cell Acute Lymphoblastic Leukemia.PMC8847596Pankaew SNozais MRemy ELoosveld MPotier DQuessada JPayet-Bornet DGrosjean CfalseCalcium Signaling Is Impaired in PTEN-Deficient T Cell Acute Lymphoblastic Leukemia.PTEN (Phosphatase and TENsin homolog) is a well-known tumor suppressor involved in numerous types of cancer, including T-cell acute lymphoblastic leukemia (T-ALL). In human, loss-of-function mutations of <i>PTEN</i> are correlated to mature T-ALL expressing a T-cell receptor (TCR) at their cell surface. In accordance with human T-ALL, inactivation of <i>Pten</i> gene in mouse thymocytes induces TCRαβ⁺ T-ALL development. Herein, we explored the functional interaction between TCRαβ signaling and PTEN. First, we performed single-cell RNA sequencing (scRNAseq) of PTEN-deficient and PTEN-proficient thymocytes. Bioinformatic analysis of our scRNAseq data showed that pathological Pten^del thymocytes express, as expected, <i>Myc</i> transcript, whereas inference of pathway act2022-01-01T00:00:00Z20222026-05-31T02:07:19.829Z2025-04-04T23:01:43.88ZS-EPMC88475963518588910.3389/fimmu.2022.797244