biostudies-literatureVera-Mansilla JAgencia Nacional de Investigación y DesarrolloANID | Fondo Nacional de Desarrollo Científico y Tecnológicoe0167821https://www.ebi.ac.uk/biostudies/studies/S-EPMC8849078biostudies-literatureUnknown10(1)Urinary tract infections (UTIs) are the second most frequent bacterial infections worldwide, with Escherichia coli being the main causative agent. The increase of antibiotic-resistance determinants among isolates from clinical samples, including UTIs, makes the development of novel therapeutic strategies a necessity. In this context, the use of bacteriophages as a therapeutic alternative has been proposed, due to their ability to efficiently kill bacteria. In this work, we isolated and characterized three novel bacteriophages, microbes laboratory phage 1 (MLP1), MLP2, and MLP3, belonging to the <i>Chaseviridae</i>, <i>Myoviridae</i>, and <i>Podoviridae</i> families, respectively. These phages efficiently infect and kill laboratory reference strains and multidrug-resistant clinical E. coli Microbiology spectrumIsolation and Characterization of Novel Lytic Phages Infecting Multidrug-Resistant Escherichia coli.PMC8849078PB-011119015811190049Sanchez PSilva-Valenzuela CAVera-Mansilla JMolina-Quiroz RCfalseIsolation and Characterization of Novel Lytic Phages Infecting Multidrug-Resistant Escherichia coli.Urinary tract infections (UTIs) are the second most frequent bacterial infections worldwide, with Escherichia coli being the main causative agent. The increase of antibiotic-resistance determinants among isolates from clinical samples, including UTIs, makes the development of novel therapeutic strategies a necessity. In this context, the use of bacteriophages as a therapeutic alternative has been proposed, due to their ability to efficiently kill bacteria. In this work, we isolated and characterized three novel bacteriophages, microbes laboratory phage 1 (MLP1), MLP2, and MLP3, belonging to the <i>Chaseviridae</i>, <i>Myoviridae</i>, and <i>Podoviridae</i> families, respectively. These phages efficiently infect and kill laboratory reference strains and multidrug-resistant clinical E. coli 2022-01-01T00:00:00Z2022 Feb2026-06-07T03:18:38.359Z2025-05-18T12:32:48.268ZS-EPMC88490783517103010.1128/spectrum.01678-21