biostudies-literatureUnknown8Molinero M<h4>Background</h4>The pathophysiology of COVID-19-related critical illness is not completely understood. Here, we analyzed the microRNA (miRNA) profile of bronchial aspirate (BAS) samples from COVID-19 and non-COVID-19 patients admitted to the ICU to identify prognostic biomarkers of fatal outcomes and to define molecular pathways involved in the disease and adverse events.<h4>Methods</h4>Two patient populations were included (<i>n</i> = 89): (i) a study population composed of critically ill COVID-19 and non-COVID-19 patients; (ii) a prospective study cohort composed of COVID-19 survivors and non-survivors among patients assisted by invasive mechanical ventilation (IMV). BAS samples were obtained by bronchoaspiration during the ICU stay. The miRNA profile was analyzed using RT-qPCR. DetaiFrontiers in medicine756517https://www.ebi.ac.uk/biostudies/studies/S-EPMC8850692biostudies-literatureBronchial Aspirate-Based Profiling Identifies MicroRNA Signatures Associated With COVID-19 and Fatal Disease in Critically Ill Patients.PMC8850692Penuelas OGort-Paniello Cde Gonzalo-Calvo DTorres GCabo RFernandez-Barat LGarcia-Gasulla DBenitez IDBermejo-Martin JFBarbe FMolinero MVengoechea JJMoncusi-Moix ACaballero JGonzalez JMotos ARiera JFerrer RGomez SRodriguez-Jara FGarcia-Hidalgo MCCeccato ATorres AMenendez RfalseBronchial Aspirate-Based Profiling Identifies MicroRNA Signatures Associated With COVID-19 and Fatal Disease in Critically Ill Patients.<h4>Background</h4>The pathophysiology of COVID-19-related critical illness is not completely understood. Here, we analyzed the microRNA (miRNA) profile of bronchial aspirate (BAS) samples from COVID-19 and non-COVID-19 patients admitted to the ICU to identify prognostic biomarkers of fatal outcomes and to define molecular pathways involved in the disease and adverse events.<h4>Methods</h4>Two patient populations were included (<i>n</i> = 89): (i) a study population composed of critically ill COVID-19 and non-COVID-19 patients; (ii) a prospective study cohort composed of COVID-19 survivors and non-survivors among patients assisted by invasive mechanical ventilation (IMV). BAS samples were obtained by bronchoaspiration during the ICU stay. The miRNA profile was analyzed using RT-qPCR. Detai2021-01-01T00:00:00Z20212025-04-29T10:59:59.824Z2025-04-06T19:49:49.91ZS-EPMC88506923518696210.3389/fmed.2021.756517