biostudies-literatureUnknown25(2)Ilina EIFNRGlioblastoma (GBM) is the most aggressive primary brain tumor characterized by infiltrative growth of malignant glioma cells into the surrounding brain parenchyma. In this study, our analysis of GBM patient cohorts revealed a significantly higher expression of <i>Glycosyltransferase</i> 8 <i>domain containing 1</i> (<i>GLT8D1</i>) compared to normal brain tissue and could be associated with impaired patient survival. Increased <i>in vitro</i> expression of <i>GLT8D1</i> significantly enhanced migration of two different sphere-forming GBM cell lines. By <i>in silico</i> analysis we predicted the 3D-structure as well as the active site residues of GLT8D1. The introduction of point mutations in the predicted active site reduced its glycosyltransferase activity <i>in vitro</i> and consequentlyiScience103842https://www.ebi.ac.uk/biostudies/studies/S-EPMC8850796biostudies-literatureEnzymatic activity of glycosyltransferase GLT8D1 promotes human glioblastoma cell migration.PMC8850796Puard VSchuster ALesur AJeanty CGerardy JJDieterle MNiclou SPMuller TGerloff DLDuarte Garcia-Escudero MMittelbronn MCialini CIlina EIMoter ADittmar GGolebiewska AThezenas MLBernardin FfalseEnzymatic activity of glycosyltransferase GLT8D1 promotes human glioblastoma cell migration.Glioblastoma (GBM) is the most aggressive primary brain tumor characterized by infiltrative growth of malignant glioma cells into the surrounding brain parenchyma. In this study, our analysis of GBM patient cohorts revealed a significantly higher expression of <i>Glycosyltransferase</i> 8 <i>domain containing 1</i> (<i>GLT8D1</i>) compared to normal brain tissue and could be associated with impaired patient survival. Increased <i>in vitro</i> expression of <i>GLT8D1</i> significantly enhanced migration of two different sphere-forming GBM cell lines. By <i>in silico</i> analysis we predicted the 3D-structure as well as the active site residues of GLT8D1. The introduction of point mutations in the predicted active site reduced its glycosyltransferase activity <i>in vitro</i> and consequently2022-01-01T00:00:00Z2022 Feb2026-05-31T08:53:36.511Z2026-04-08T08:12:37.014ZS-EPMC88507963519889510.1016/j.isci.2022.103842