biostudies-literatureInaba Kjapan agency for medical research and developmentjapan society for the promotion of science43https://www.ebi.ac.uk/biostudies/studies/S-EPMC8851771biostudies-literatureUnknown20(1)<h4>Background</h4>Mosquito control is a crucial global issue for protecting the human community from mosquito-borne diseases. There is an urgent need for the development of selective and safe reagents for mosquito control. Flavonoids, a group of chemical substances with variable phenolic structures, such as daidzein, have been suggested as potential mosquito larvicides with less risk to the environment. However, the mode of mosquito larvicidal action of flavonoids has not been elucidated.<h4>Results</h4>Here, we report that several flavonoids, including daidzein, inhibit the activity of glutathione S-transferase Noppera-bo (Nobo), an enzyme used for the biosynthesis of the insect steroid hormone ecdysone, in the yellow fever mosquito Aedes aegypti. The crystal structure of the Nobo proteiBMC biologyMolecular action of larvicidal flavonoids on ecdysteroidogenic glutathione S-transferase Noppera-bo in Aedes aegypti.PMC885177117H01472JP21am0101086JP21am010107118K19163JP21am010111415K14719JP21am0101113Honma TFukuzawa KHirokawa TInaba KEbihara KTakaya DUemura NWatanabe ANiwa RWatanabe CKawashima YSenda TKojima HWatanabe KSenda MNishimura TImamura RKasai SInoue HFujikawa YKoiwai KSakuma CKanuka HOkabe TYoshino RfalseMolecular action of larvicidal flavonoids on ecdysteroidogenic glutathione S-transferase Noppera-bo in Aedes aegypti.<h4>Background</h4>Mosquito control is a crucial global issue for protecting the human community from mosquito-borne diseases. There is an urgent need for the development of selective and safe reagents for mosquito control. Flavonoids, a group of chemical substances with variable phenolic structures, such as daidzein, have been suggested as potential mosquito larvicides with less risk to the environment. However, the mode of mosquito larvicidal action of flavonoids has not been elucidated.<h4>Results</h4>Here, we report that several flavonoids, including daidzein, inhibit the activity of glutathione S-transferase Noppera-bo (Nobo), an enzyme used for the biosynthesis of the insect steroid hormone ecdysone, in the yellow fever mosquito Aedes aegypti. The crystal structure of the Nobo protei2022-01-01T00:00:00Z2022 Feb2026-05-31T07:05:57.171Z2025-02-19T05:04:43.319ZS-EPMC88517713517281610.1186/s12915-022-01233-2