{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["de Oliveira RC"],"funding":["NEI NIH HHS","NIH HHS"],"pagination":["50-60"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8852807"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["38(1)"],"pubmed_abstract":["<h4>Purpose</h4>To study epithelial basement membrane (EBM) regeneration in non-fibrotic and fibrotic corneas after photorefractive keratectomy (PRK).<h4>Methods</h4>Rabbits (120 total) had either epithelial scrape alone, -4.50 diopters (D) PRK, -9.00 D PRK, or no surgery. Immunohistochemistry was performed on cryofixed corneas at time points from unwounded to 8 weeks (four corneas at each time point in each group). Multiplex immunohistochemistry was performed for EBM components, including collagen type IV, laminin beta-3, laminin alpha-5, perlecan, and nidogen-1. Stromal cellular composition was studied by triplex immunohistochemistry for keratocan, vimentin, and alpha-smooth muscle actin (SMA).<h4>Results</h4>PRK-injured EBM significantly regenerated by 4 days after surgery. However, ear"],"journal":["Journal of refractive surgery (Thorofare, N.J. : 1995)"],"pubmed_title":["Epithelial Basement Membrane Regeneration After PRK-Induced Epithelial-Stromal Injury in Rabbits: Fibrotic Versus Non-fibrotic Corneal Healing."],"pmcid":["PMC8852807"],"funding_grant_id":["R01 EY010056","P30 EY025585","S10 OD019972"],"pubmed_authors":["Wilson SE","Sampaio LP","Shiju TM","de Oliveira RC","Santhiago MR"],"additional_accession":[]},"is_claimable":false,"name":"Epithelial Basement Membrane Regeneration After PRK-Induced Epithelial-Stromal Injury in Rabbits: Fibrotic Versus Non-fibrotic Corneal Healing.","description":"<h4>Purpose</h4>To study epithelial basement membrane (EBM) regeneration in non-fibrotic and fibrotic corneas after photorefractive keratectomy (PRK).<h4>Methods</h4>Rabbits (120 total) had either epithelial scrape alone, -4.50 diopters (D) PRK, -9.00 D PRK, or no surgery. Immunohistochemistry was performed on cryofixed corneas at time points from unwounded to 8 weeks (four corneas at each time point in each group). Multiplex immunohistochemistry was performed for EBM components, including collagen type IV, laminin beta-3, laminin alpha-5, perlecan, and nidogen-1. Stromal cellular composition was studied by triplex immunohistochemistry for keratocan, vimentin, and alpha-smooth muscle actin (SMA).<h4>Results</h4>PRK-injured EBM significantly regenerated by 4 days after surgery. However, ear","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2026-04-07T14:59:15.159Z","creation":"2026-04-07T14:24:31.162Z"},"accession":"S-EPMC8852807","cross_references":{"pubmed":["35020537"],"doi":["10.3928/1081597X-20211007-02","10.3928/1081597x-20211007-02"]}}