biostudies-literaturede Oliveira RCNEI NIH HHSNIH HHS50-60https://www.ebi.ac.uk/biostudies/studies/S-EPMC8852807biostudies-literatureUnknown38(1)<h4>Purpose</h4>To study epithelial basement membrane (EBM) regeneration in non-fibrotic and fibrotic corneas after photorefractive keratectomy (PRK).<h4>Methods</h4>Rabbits (120 total) had either epithelial scrape alone, -4.50 diopters (D) PRK, -9.00 D PRK, or no surgery. Immunohistochemistry was performed on cryofixed corneas at time points from unwounded to 8 weeks (four corneas at each time point in each group). Multiplex immunohistochemistry was performed for EBM components, including collagen type IV, laminin beta-3, laminin alpha-5, perlecan, and nidogen-1. Stromal cellular composition was studied by triplex immunohistochemistry for keratocan, vimentin, and alpha-smooth muscle actin (SMA).<h4>Results</h4>PRK-injured EBM significantly regenerated by 4 days after surgery. However, earJournal of refractive surgery (Thorofare, N.J. : 1995)Epithelial Basement Membrane Regeneration After PRK-Induced Epithelial-Stromal Injury in Rabbits: Fibrotic Versus Non-fibrotic Corneal Healing.PMC8852807R01 EY010056P30 EY025585S10 OD019972Wilson SESampaio LPShiju TMde Oliveira RCSanthiago MRfalseEpithelial Basement Membrane Regeneration After PRK-Induced Epithelial-Stromal Injury in Rabbits: Fibrotic Versus Non-fibrotic Corneal Healing.<h4>Purpose</h4>To study epithelial basement membrane (EBM) regeneration in non-fibrotic and fibrotic corneas after photorefractive keratectomy (PRK).<h4>Methods</h4>Rabbits (120 total) had either epithelial scrape alone, -4.50 diopters (D) PRK, -9.00 D PRK, or no surgery. Immunohistochemistry was performed on cryofixed corneas at time points from unwounded to 8 weeks (four corneas at each time point in each group). Multiplex immunohistochemistry was performed for EBM components, including collagen type IV, laminin beta-3, laminin alpha-5, perlecan, and nidogen-1. Stromal cellular composition was studied by triplex immunohistochemistry for keratocan, vimentin, and alpha-smooth muscle actin (SMA).<h4>Results</h4>PRK-injured EBM significantly regenerated by 4 days after surgery. However, ear2022-01-01T00:00:00Z2022 Jan2026-04-07T14:59:15.159Z2026-04-07T14:24:31.162ZS-EPMC88528073502053710.3928/1081597X-20211007-0210.3928/1081597x-20211007-02