biostudies-literatureUnknown16(3)Fodelianakis SNOMIS StiftungGlacier-fed streams (GFSs) are extreme and rapidly vanishing ecosystems, and yet they harbor diverse microbial communities. Although our understanding of the GFS microbiome has recently increased, we do not know which microbial clades are ecologically successful in these ecosystems, nor do we understand potentially underlying mechanisms. Ecologically successful clades should be more prevalent across GFSs compared to other clades, which should be reflected as clade-wise distinctly low phylogenetic turnover. However, methods to assess such patterns are currently missing. Here we developed and applied a novel analytical framework, "phyloscore analysis", to identify clades with lower spatial phylogenetic turnover than other clades in the sediment microbiome across twenty GFSs in New Zealand. TThe ISME journal666-675https://www.ebi.ac.uk/biostudies/studies/S-EPMC8857233biostudies-literatureMicrodiversity characterizes prevalent phylogenetic clades in the glacier-fed stream microbiome.PMC8857233Bourquin MBusi SBStyllas MDe Staercke VTolosano MPramateftaki PSchon MFodelianakis SKohler TJBattin TJBrandani JPeter HWashburne ADWilmes PfalseMicrodiversity characterizes prevalent phylogenetic clades in the glacier-fed stream microbiome.Glacier-fed streams (GFSs) are extreme and rapidly vanishing ecosystems, and yet they harbor diverse microbial communities. Although our understanding of the GFS microbiome has recently increased, we do not know which microbial clades are ecologically successful in these ecosystems, nor do we understand potentially underlying mechanisms. Ecologically successful clades should be more prevalent across GFSs compared to other clades, which should be reflected as clade-wise distinctly low phylogenetic turnover. However, methods to assess such patterns are currently missing. Here we developed and applied a novel analytical framework, "phyloscore analysis", to identify clades with lower spatial phylogenetic turnover than other clades in the sediment microbiome across twenty GFSs in New Zealand. T2022-01-01T00:00:00Z2022 Mar2026-05-09T09:37:25.063Z2025-04-19T23:33:59.917ZS-EPMC88572333452200910.1038/s41396-021-01106-6