{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["27"],"submitter":["Valentini P"],"pubmed_abstract":["SINEUPs are a novel class of natural and synthetic non-coding antisense RNA molecules able to increase the translation of a target mRNA. They present a modular organization comprising an unstructured antisense target-specific domain, which sets the specificity of each individual SINEUP, and a structured effector domain, which is responsible for the translation enhancement. In order to design a fully functional <i>in vitro</i> transcribed SINEUP for therapeutics applications, SINEUP RNAs were synthesized <i>in vitro</i> with a variety of chemical modifications and screened for their activity on endogenous target mRNA upon transfection. Three combinations of modified ribonucleotides-2'O methyl-ATP (Am), N6 methyl-ATP (m6A), and pseudo-UTP (ψ)-conferred SINEUP activity to naked RNA. The best "],"journal":["Molecular therapy. Nucleic acids"],"pagination":["1092-1102"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8857549"],"repository":["biostudies-literature"],"pubmed_title":["Towards SINEUP-based therapeutics: Design of an <i>in vitro</i> synthesized SINEUP RNA."],"pmcid":["PMC8857549"],"pubmed_authors":["Valentini P","Zacco E","Mangoni D","Carninci P","Espinoza S","Andrews B","Webster NA","Gustincich S","Pandolfini L","Pierattini B","Tartaglia GG"],"additional_accession":[]},"is_claimable":false,"name":"Towards SINEUP-based therapeutics: Design of an <i>in vitro</i> synthesized SINEUP RNA.","description":"SINEUPs are a novel class of natural and synthetic non-coding antisense RNA molecules able to increase the translation of a target mRNA. They present a modular organization comprising an unstructured antisense target-specific domain, which sets the specificity of each individual SINEUP, and a structured effector domain, which is responsible for the translation enhancement. In order to design a fully functional <i>in vitro</i> transcribed SINEUP for therapeutics applications, SINEUP RNAs were synthesized <i>in vitro</i> with a variety of chemical modifications and screened for their activity on endogenous target mRNA upon transfection. Three combinations of modified ribonucleotides-2'O methyl-ATP (Am), N6 methyl-ATP (m6A), and pseudo-UTP (ψ)-conferred SINEUP activity to naked RNA. The best ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-22T04:44:53.288Z","creation":"2025-04-05T21:02:54.971Z"},"accession":"S-EPMC8857549","cross_references":{"pubmed":["35228902"],"doi":["10.1016/j.omtn.2022.01.021"]}}