biostudies-literatureUnknown8Wang LBrowning seriously affects the quality of fresh-cut fruits, and its mechanism was thought to be polyphenol oxidase (PPO) in the past. A way of non-different PPO browning was found in our previous studies. However, the landscape of this browning way is still unclear in "Fuji" apples. Multi-omics (methylomics, transcriptomics, and proteomics) methods were performed to the global profiles of DNA methylation and gene and protein expression. We employed two natural bud mutation varieties of apple as materials and found a positive correlation between browning index (BI) and methylation (5mC%, MdCMT3, and MdCMT3c) and a negative correlation between BI and demethylation (MdROS1 and MdDME). DNA methylation inhibitor 5-azacytidine can delay apple browning. Further analysiFrontiers in nutrition800489https://www.ebi.ac.uk/biostudies/studies/S-EPMC8859415biostudies-literatureMulti-Omics Landscape of DNA Methylation Regulates Browning in "Fuji" Apple.PMC8859415Zhang JWang FWang WTang TWang ZWang LfalseMulti-Omics Landscape of DNA Methylation Regulates Browning in "Fuji" Apple.Browning seriously affects the quality of fresh-cut fruits, and its mechanism was thought to be polyphenol oxidase (PPO) in the past. A way of non-different PPO browning was found in our previous studies. However, the landscape of this browning way is still unclear in "Fuji" apples. Multi-omics (methylomics, transcriptomics, and proteomics) methods were performed to the global profiles of DNA methylation and gene and protein expression. We employed two natural bud mutation varieties of apple as materials and found a positive correlation between browning index (BI) and methylation (5mC%, MdCMT3, and MdCMT3c) and a negative correlation between BI and demethylation (MdROS1 and MdDME). DNA methylation inhibitor 5-azacytidine can delay apple browning. Further analysi2021-01-01T00:00:00Z20212025-04-05T13:07:55.925Z2025-04-05T13:07:55.925ZS-EPMC88594153519858510.3389/fnut.2021.800489