biostudies-literatureNaveca FGLNCCMCTI | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa e Inovação do Espírito Santo (FAPES)WHO | Pan American Health Organization (PAHO)WHO | Pan American Health OrganizationFundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM)Fundação de Amparo à Pesquisa e Inovação do Espírito SantoFundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)MCTI | Conselho Nacional de Desenvolvimento Científico e TecnológicoFundação Oswaldo CruzFundação Oswaldo Cruz (FIOCRUZ)Fundação de Amparo à Pesquisa do Estado do AmazonasFundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiroe0236621https://www.ebi.ac.uk/biostudies/studies/S-EPMC8865440biostudies-literatureUnknown10(1)The Amazonas was one of the most heavily affected Brazilian states by the COVID-19 epidemic. Despite a large number of infected people, particularly during the second wave associated with the spread of the Variant of Concern (VOC) Gamma (lineage P.1), SARS-CoV-2 continues to circulate in the Amazonas. To understand how SARS-CoV-2 persisted in a human population with a high immunity barrier, we generated 1,188 SARS-CoV-2 whole-genome sequences from individuals diagnosed in the Amazonas state from 1st January to 6th July 2021, of which 38 were vaccine breakthrough infections. Our study reveals a sharp increase in the relative prevalence of Gamma plus (P.1+) variants, designated Pango Lineages P.1.3 to P.1.6, harboring two types of additional Spike changes: deletions in the N-terminal (NTD) dMicrobiology spectrumSpread of Gamma (P.1) Sub-Lineages Carrying Spike Mutations Close to the Furin Cleavage Site and Deletions in the N-Terminal Domain Drives Ongoing Transmission of SARS-CoV-2 in Amazonas, Brazil.PMC8865440deE-26/202.896/2018mica de VigilâVPPCB-007-FIO-18-2-30157/2021403276/2020-9ncia em SaúPCTI-EmergeSaudeRede GenôMartins ACosta ANaveca FGCorado ALMejia MCBello GFiocruz COVID-19 Genomic Surveillance NetworkPessoa KDos Santos ERResende PCde Melo GCMiyajima FMourao MPDabilla NXavier MSBatista ELRNascimento VBrandao MJGoncalves LSampaio VSCoelho DFerraz MAbdalla LDias FBSLacerda MVSiqueira MMWallau GLNascimento FSouza VJesus MVinhal FFernandes CSilva GMattos TSantos JHChui FMVal FFMagalhaes ALAGraf TDelatorre EDuarte DPereira LCGLins RfalseSpread of Gamma (P.1) Sub-Lineages Carrying Spike Mutations Close to the Furin Cleavage Site and Deletions in the N-Terminal Domain Drives Ongoing Transmission of SARS-CoV-2 in Amazonas, Brazil.The Amazonas was one of the most heavily affected Brazilian states by the COVID-19 epidemic. Despite a large number of infected people, particularly during the second wave associated with the spread of the Variant of Concern (VOC) Gamma (lineage P.1), SARS-CoV-2 continues to circulate in the Amazonas. To understand how SARS-CoV-2 persisted in a human population with a high immunity barrier, we generated 1,188 SARS-CoV-2 whole-genome sequences from individuals diagnosed in the Amazonas state from 1st January to 6th July 2021, of which 38 were vaccine breakthrough infections. Our study reveals a sharp increase in the relative prevalence of Gamma plus (P.1+) variants, designated Pango Lineages P.1.3 to P.1.6, harboring two types of additional Spike changes: deletions in the N-terminal (NTD) d2022-01-01T00:00:00Z2022 Feb2026-06-07T06:02:46.182Z2025-05-29T20:10:57.361ZS-EPMC88654403519678310.1128/spectrum.02366-21