biostudies-literatureZeng Qthe Innovation-Driven Project of Central South University, ChinaNational Natural Science Foundation of China307https://www.ebi.ac.uk/biostudies/studies/S-EPMC8868171biostudies-literatureUnknown11(2)Idiopathic pulmonary fibrosis (IPF) can severely disrupt lung function, leading to fatal consequences, and there is currently a lack of specific therapeutic drugs. Bergenin is an isocoumarin compound with lots of biological functions including antioxidant activity. This study evaluated the potential beneficial effects of bergenin on pulmonary fibrosis and investigated the possible mechanisms. We found that bergenin alleviated bleomycin-induced pulmonary fibrosis by relieving oxidative stress, reducing the deposition of the extracellular matrix (ECM) and inhibiting the formation of myofibroblasts. Furthermore, we showed that bergenin could induce phosphorylation and expression of p62 and activation of Nrf2, Nrf2 was required for bergenin-induced p62 upregulation, and p62 knockdown reduced bAntioxidants (Basel, Switzerland)p62-Nrf2 Regulatory Loop Mediates the Anti-Pulmonary Fibrosis Effect of Bergenin.PMC88681712017TP1004S2021105330490821708532016JJ318282100084815000562020JJ47732021JJ30889Xie WZeng QChen JLuo ZHua QZhou TDeng LLiu WSang XRen LHuang XTang SZhao FXiong DHe BWang ZLin Mfalsep62-Nrf2 Regulatory Loop Mediates the Anti-Pulmonary Fibrosis Effect of Bergenin.Idiopathic pulmonary fibrosis (IPF) can severely disrupt lung function, leading to fatal consequences, and there is currently a lack of specific therapeutic drugs. Bergenin is an isocoumarin compound with lots of biological functions including antioxidant activity. This study evaluated the potential beneficial effects of bergenin on pulmonary fibrosis and investigated the possible mechanisms. We found that bergenin alleviated bleomycin-induced pulmonary fibrosis by relieving oxidative stress, reducing the deposition of the extracellular matrix (ECM) and inhibiting the formation of myofibroblasts. Furthermore, we showed that bergenin could induce phosphorylation and expression of p62 and activation of Nrf2, Nrf2 was required for bergenin-induced p62 upregulation, and p62 knockdown reduced b2022-01-01T00:00:00Z2022 Feb2025-04-04T07:55:05.34Z2025-02-19T05:05:01.113ZS-EPMC88681713520419010.3390/antiox11020307