{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(2)"],"submitter":["Murugesan G"],"pubmed_abstract":["Macrophages (MΦ) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated MΦ can exist in two extreme phenotypes: pro-inflammatory (M1) MΦ and anti-inflammatory (M2) MΦ. These phenotypes can be recapitulated in vitro by using ligands of toll-like receptors (TLRs) and cytokines such as IFNγ and IL-4. In recent years, human induced pluripotent stem cells (iPSC)-derived MΦ have gained major attention, as they are functionally similar to human monocyte-derived MΦ and are receptive to genome editing. In this study, we polarised iPSC-derived MΦ to M1 or M2 and analysed their proteome and secretome profiles using quantitative proteomics. These comprehensive proteomic data sets provide new insights into functions of polarised MΦ."],"journal":["Biomedicines"],"pagination":["239"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8869710"],"repository":["biostudies-literature"],"pubmed_title":["Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells."],"pmcid":["PMC8869710"],"pubmed_authors":["Weigle B","Murugesan G","Crocker PR","Davidson L","Jannetti L"],"additional_accession":[]},"is_claimable":false,"name":"Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells.","description":"Macrophages (MΦ) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated MΦ can exist in two extreme phenotypes: pro-inflammatory (M1) MΦ and anti-inflammatory (M2) MΦ. These phenotypes can be recapitulated in vitro by using ligands of toll-like receptors (TLRs) and cytokines such as IFNγ and IL-4. In recent years, human induced pluripotent stem cells (iPSC)-derived MΦ have gained major attention, as they are functionally similar to human monocyte-derived MΦ and are receptive to genome editing. In this study, we polarised iPSC-derived MΦ to M1 or M2 and analysed their proteome and secretome profiles using quantitative proteomics. These comprehensive proteomic data sets provide new insights into functions of polarised MΦ.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2025-04-22T03:49:05.945Z","creation":"2025-04-05T20:50:16.325Z"},"accession":"S-EPMC8869710","cross_references":{"pubmed":["35203449"],"doi":["10.3390/biomedicines10020239"]}}