biostudies-literatureUnknown10(2)Murugesan GMacrophages (MΦ) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated MΦ can exist in two extreme phenotypes: pro-inflammatory (M1) MΦ and anti-inflammatory (M2) MΦ. These phenotypes can be recapitulated in vitro by using ligands of toll-like receptors (TLRs) and cytokines such as IFNγ and IL-4. In recent years, human induced pluripotent stem cells (iPSC)-derived MΦ have gained major attention, as they are functionally similar to human monocyte-derived MΦ and are receptive to genome editing. In this study, we polarised iPSC-derived MΦ to M1 or M2 and analysed their proteome and secretome profiles using quantitative proteomics. These comprehensive proteomic data sets provide new insights into functions of polarised MΦ.Biomedicines239https://www.ebi.ac.uk/biostudies/studies/S-EPMC8869710biostudies-literatureQuantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells.PMC8869710Weigle BMurugesan GCrocker PRDavidson LJannetti LfalseQuantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells.Macrophages (MΦ) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated MΦ can exist in two extreme phenotypes: pro-inflammatory (M1) MΦ and anti-inflammatory (M2) MΦ. These phenotypes can be recapitulated in vitro by using ligands of toll-like receptors (TLRs) and cytokines such as IFNγ and IL-4. In recent years, human induced pluripotent stem cells (iPSC)-derived MΦ have gained major attention, as they are functionally similar to human monocyte-derived MΦ and are receptive to genome editing. In this study, we polarised iPSC-derived MΦ to M1 or M2 and analysed their proteome and secretome profiles using quantitative proteomics. These comprehensive proteomic data sets provide new insights into functions of polarised MΦ.2022-01-01T00:00:00Z2022 Jan2025-04-22T03:49:05.945Z2025-04-05T20:50:16.325ZS-EPMC88697103520344910.3390/biomedicines10020239