biostudies-literatureChen YCMinistry of Science and TechnologyNational Sun Yat-sen University-KMU Joint Research ProjectKaohsiung Medical University HospitalKaohsiung Medical University Research Center641https://www.ebi.ac.uk/biostudies/studies/S-EPMC8870566biostudies-literatureUnknown11(4)Cryptocaryone (CPC) is a bioactive dihydrochalcone derived from <i>Cryptocarya</i> plants, and its antiproliferation was rarely reported, especially for ovarian cancer (OVCA). This study aimed to examine the regulation ability and mechanism of CPC on three histotypes of OVCA cells (SKOV3, TOV-21G, and TOV-112D). In a 24 h MTS assay, CPC showed antiproliferation effects to OVCA cells, i.e., IC₅₀ values 1.5, 3, and 9.5 μM for TOV-21G, SKOV3, and TOV-112D cells. TOV-21G and SKOV3 cells showed hypersensitivity to CPC when applied for exposure time and concentration experiments. For biological processes, CPC stimulated the generation of reactive oxygen species and mitochondrial superoxide and promoted mitochondrial membrane potential dysfunction in TOV-21G and SKOV3 cells. Apoptosis CellsCryptocaryone Promotes ROS-Dependent Antiproliferation and Apoptosis in Ovarian Cancer Cells.PMC8870566KMUH110-0R72MOST 108-2314-B-037-021-MY3; MOST 108-2320-B-037-015-MY3; MOST 110-2314-B-037-075-MY2KMU-TC108A04#NSYSUKMU 111-P20Chang HWChan TFYen CHChen YCChang HSYang CWFarooqi AAHuang MYfalseCryptocaryone Promotes ROS-Dependent Antiproliferation and Apoptosis in Ovarian Cancer Cells.Cryptocaryone (CPC) is a bioactive dihydrochalcone derived from <i>Cryptocarya</i> plants, and its antiproliferation was rarely reported, especially for ovarian cancer (OVCA). This study aimed to examine the regulation ability and mechanism of CPC on three histotypes of OVCA cells (SKOV3, TOV-21G, and TOV-112D). In a 24 h MTS assay, CPC showed antiproliferation effects to OVCA cells, i.e., IC₅₀ values 1.5, 3, and 9.5 μM for TOV-21G, SKOV3, and TOV-112D cells. TOV-21G and SKOV3 cells showed hypersensitivity to CPC when applied for exposure time and concentration experiments. For biological processes, CPC stimulated the generation of reactive oxygen species and mitochondrial superoxide and promoted mitochondrial membrane potential dysfunction in TOV-21G and SKOV3 cells. Apoptosis 2022-01-01T00:00:00Z2022 Feb2025-04-26T19:02:30.91Z2025-04-06T16:01:19.003ZS-EPMC88705663520329410.3390/cells11040641