biostudies-literatureVidor CJAustralian Research Council124https://www.ebi.ac.uk/biostudies/studies/S-EPMC8878733biostudies-literatureUnknown14(2)Protein secretion is generally mediated by a series of distinct pathways in bacteria. Recently, evidence of a novel bacterial secretion pathway involving a bacteriophage-related protein has emerged. TcdE, a holin-like protein encoded by toxigenic isolates of <i>Clostridioides difficile</i>, mediates the release of the large clostridial glucosylating toxins (LCGTs), TcdA and TcdB, and TpeL from <i>C. perfringens</i> uses another holin-like protein, TpeE, for its secretion; however, it is not yet known if TcdE or TpeE secretion is specific to these proteins. It is also unknown if other members of the LCGT-producing clostridia, including <i>Paeniclostridium sordellii</i> (previously <i>Clostridium sordellii</i>)<i>,</i> use a similar toxin-release mechanism. Here, we confirm that each of the ToxinsA Highly Specific Holin-Mediated Mechanism Facilitates the Secretion of Lethal Toxin TcsL in <i>Paeniclostridium</i><i>sordellii</i>.PMC8878733FT120100779Mathias RAAwad MLyras DDupuy BHamiot AWisniewski JVidor CJfalseA Highly Specific Holin-Mediated Mechanism Facilitates the Secretion of Lethal Toxin TcsL in <i>Paeniclostridium</i><i>sordellii</i>.Protein secretion is generally mediated by a series of distinct pathways in bacteria. Recently, evidence of a novel bacterial secretion pathway involving a bacteriophage-related protein has emerged. TcdE, a holin-like protein encoded by toxigenic isolates of <i>Clostridioides difficile</i>, mediates the release of the large clostridial glucosylating toxins (LCGTs), TcdA and TcdB, and TpeL from <i>C. perfringens</i> uses another holin-like protein, TpeE, for its secretion; however, it is not yet known if TcdE or TpeE secretion is specific to these proteins. It is also unknown if other members of the LCGT-producing clostridia, including <i>Paeniclostridium sordellii</i> (previously <i>Clostridium sordellii</i>)<i>,</i> use a similar toxin-release mechanism. Here, we confirm that each of the 2022-01-01T00:00:00Z2022 Feb2026-04-08T17:56:08.473Z2024-11-13T18:13:05.615ZS-EPMC88787333520215110.3390/toxins14020124