biostudies-literatureBerger NInstitut national de la recherche pour l'agriculture, l'alimentation et l'environnementInstitut national de la recherche pour l'agriculture, l'alimentation et l'environnement1956https://www.ebi.ac.uk/biostudies/studies/S-EPMC8879470biostudies-literatureUnknown23(4)Osmotic stress can be detrimental to plants, whose survival relies heavily on proteomic plasticity. Protein ubiquitination is a central post-translational modification in osmotic-mediated stress. In this study, we used the K-Ɛ-GG antibody enrichment method integrated with high-resolution mass spectrometry to compile a list of 719 ubiquitinated lysine (K-Ub) residues from 450 Arabidopsis root membrane proteins (58% of which are transmembrane proteins), thereby adding to the database of ubiquitinated substrates in plants. Although no ubiquitin (Ub) motifs could be identified, the presence of acidic residues close to K-Ub was revealed. Our ubiquitinome analysis pointed to a broad role of ubiquitination in the internalization and sorting of cargo proteins. Moreover, the simultaneous proteome aInternational journal of molecular sciencesRoot Membrane Ubiquitinome under Short-Term Osmotic Stress.PMC8879470no numberRofidal VCrabos ANacry PBerger NHem SDemolombe VSteinmann LKrouk GVerdoucq LSantoni VfalseRoot Membrane Ubiquitinome under Short-Term Osmotic Stress.Osmotic stress can be detrimental to plants, whose survival relies heavily on proteomic plasticity. Protein ubiquitination is a central post-translational modification in osmotic-mediated stress. In this study, we used the K-Ɛ-GG antibody enrichment method integrated with high-resolution mass spectrometry to compile a list of 719 ubiquitinated lysine (K-Ub) residues from 450 Arabidopsis root membrane proteins (58% of which are transmembrane proteins), thereby adding to the database of ubiquitinated substrates in plants. Although no ubiquitin (Ub) motifs could be identified, the presence of acidic residues close to K-Ub was revealed. Our ubiquitinome analysis pointed to a broad role of ubiquitination in the internalization and sorting of cargo proteins. Moreover, the simultaneous proteome a2022-01-01T00:00:00Z2022 Feb2026-04-08T17:53:34.585Z2025-02-19T05:04:54.156ZS-EPMC88794703521607410.3390/ijms23041956