{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Seo JH"],"funding":["Rural Development Administration"],"pagination":["2382"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8880809"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(4)"],"pubmed_abstract":["Pod-shattering causes a significant yield loss in many soybean cultivars. Shattering-tolerant cultivars provide the most effective approach to minimizing this loss. We developed molecular markers for pod-shattering and validated them in soybeans with diverse genetic backgrounds. The genes Glyma.16g141200, Glyma.16g141500, and Glyma.16g076600, identified in our previous study by quantitative trait locus (QTL) mapping and whole-genome resequencing, were selected for marker development. The whole-genome resequencing of three parental lines (one shattering-tolerant and two shattering-susceptible) identified single nucleotide polymorphism (SNP) and/or insertion/deletion (InDel) regions within or near the selected genes. Two SNPs and one InDel were converted to Kompetitive Allele-Specific PCR (K"],"journal":["International journal of molecular sciences"],"pubmed_title":["Development and Validation of SNP and InDel Markers for Pod-Shattering Tolerance in Soybean."],"pmcid":["PMC8880809"],"funding_grant_id":["PJ01186803"],"pubmed_authors":["Seo JH","Sung JS","Ko JY","Jun TH","Baek IY","Kang BK","Kim KS","Jung CS","Dhungana SK"],"additional_accession":[]},"is_claimable":false,"name":"Development and Validation of SNP and InDel Markers for Pod-Shattering Tolerance in Soybean.","description":"Pod-shattering causes a significant yield loss in many soybean cultivars. Shattering-tolerant cultivars provide the most effective approach to minimizing this loss. We developed molecular markers for pod-shattering and validated them in soybeans with diverse genetic backgrounds. The genes Glyma.16g141200, Glyma.16g141500, and Glyma.16g076600, identified in our previous study by quantitative trait locus (QTL) mapping and whole-genome resequencing, were selected for marker development. The whole-genome resequencing of three parental lines (one shattering-tolerant and two shattering-susceptible) identified single nucleotide polymorphism (SNP) and/or insertion/deletion (InDel) regions within or near the selected genes. Two SNPs and one InDel were converted to Kompetitive Allele-Specific PCR (K","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Feb","modification":"2025-04-04T14:59:07.599Z","creation":"2025-04-04T14:59:07.599Z"},"accession":"S-EPMC8880809","cross_references":{"pubmed":["35216500"],"doi":["10.3390/ijms23042382"]}}