{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["He F"],"funding":["National Center for Advancing Translational Sciences","University of Chicago Orthopaedics Alumni Fund","NCATS NIH HHS","Mabel Green Myers Research Endowment Fund","University of Chicago Cancer Center","NCI NIH HHS","National Institutes of Health","NIGMS NIH HHS","SHOCK Fund"],"pagination":["e22"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8887422"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["50(4)"],"pubmed_abstract":["MicroRNAs (miRNAs or miRs) are single-stranded, ∼22-nucleotide noncoding RNAs that regulate many cellular processes. While numerous miRNA quantification technologies are available, a recent analysis of 12 commercial platforms revealed high variations in reproducibility, sensitivity, accuracy, specificity and concordance within and/or between platforms. Here, we developed a universal hairpin primer (UHP) system that negates the use of miRNA-specific hairpin primers (MsHPs) for quantitative reverse transcription PCR (RT-qPCR)-based miRNA quantification. Specifically, we analyzed four UHPs that share the same hairpin structure but are anchored with two, three, four and six degenerate nucleotides at 3'-ends (namely UHP2, UHP3, UHP4 and UHP6), and found that the four UHPs yielded robust RT prod"],"journal":["Nucleic acids research"],"pubmed_title":["OUHP: an optimized universal hairpin primer system for cost-effective and high-throughput RT-qPCR-based quantification of microRNA (miRNA) expression."],"pmcid":["PMC8887422"],"funding_grant_id":["UL1 TR000430","P30CA014599","DE030480","CA226303","T32 GM007281","P30 CA014599"],"pubmed_authors":["Xia Y","He F","Shen L","Luu HH","Ho SH","Zeng Z","Hendren-Santiago B","Gan H","Wang H","Ni N","Chen C","Qin KH","Fan J","Zhao P","He TC","Wagstaff W","Haydon RC","Shi D","Hu DA","Qin D","Reid RR"],"additional_accession":[]},"is_claimable":false,"name":"OUHP: an optimized universal hairpin primer system for cost-effective and high-throughput RT-qPCR-based quantification of microRNA (miRNA) expression.","description":"MicroRNAs (miRNAs or miRs) are single-stranded, ∼22-nucleotide noncoding RNAs that regulate many cellular processes. While numerous miRNA quantification technologies are available, a recent analysis of 12 commercial platforms revealed high variations in reproducibility, sensitivity, accuracy, specificity and concordance within and/or between platforms. Here, we developed a universal hairpin primer (UHP) system that negates the use of miRNA-specific hairpin primers (MsHPs) for quantitative reverse transcription PCR (RT-qPCR)-based miRNA quantification. Specifically, we analyzed four UHPs that share the same hairpin structure but are anchored with two, three, four and six degenerate nucleotides at 3'-ends (namely UHP2, UHP3, UHP4 and UHP6), and found that the four UHPs yielded robust RT prod","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Feb","modification":"2025-04-26T04:39:34.251Z","creation":"2025-04-06T11:12:45.015Z"},"accession":"S-EPMC8887422","cross_references":{"pubmed":["34850128"],"doi":["10.1093/nar/gkab1153"]}}