{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ball CB"],"funding":["National Institutes of Health","NIGMS NIH HHS"],"pagination":["1908-1926"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8887472"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["50(4)"],"pubmed_abstract":["Approximately half of purified mammalian RNA polymerase II (Pol II) is associated with a tightly interacting sub-stoichiometric subunit, Gdown1. Previous studies have established that Gdown1 inhibits transcription initiation through competitive interactions with general transcription factors and blocks the Pol II termination activity of transcription termination factor 2 (TTF2). However, the biological functions of Gdown1 remain poorly understood. Here, we utilized genetic, microscopic, and multi-omics approaches to functionally characterize Gdown1 in three human cell lines. Acute depletion of Gdown1 caused minimal direct effects on transcription. We show that Gdown1 resides predominantly in the cytoplasm of interphase cells, shuttles between the cytoplasm and nucleus, and is regulated by "],"journal":["Nucleic acids research"],"pubmed_title":["Nuclear export restricts Gdown1 to a mitotic function."],"pmcid":["PMC8887472"],"funding_grant_id":["R35 GM126908"],"pubmed_authors":["Ball CB","Suarez GA","Price DH","Parida M","Spector BM","Santana JF"],"additional_accession":[]},"is_claimable":false,"name":"Nuclear export restricts Gdown1 to a mitotic function.","description":"Approximately half of purified mammalian RNA polymerase II (Pol II) is associated with a tightly interacting sub-stoichiometric subunit, Gdown1. Previous studies have established that Gdown1 inhibits transcription initiation through competitive interactions with general transcription factors and blocks the Pol II termination activity of transcription termination factor 2 (TTF2). However, the biological functions of Gdown1 remain poorly understood. Here, we utilized genetic, microscopic, and multi-omics approaches to functionally characterize Gdown1 in three human cell lines. Acute depletion of Gdown1 caused minimal direct effects on transcription. We show that Gdown1 resides predominantly in the cytoplasm of interphase cells, shuttles between the cytoplasm and nucleus, and is regulated by ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Feb","modification":"2026-04-08T18:18:32.277Z","creation":"2025-04-19T22:37:45.678Z"},"accession":"S-EPMC8887472","cross_references":{"pubmed":["35048979"],"doi":["10.1093/nar/gkac015"]}}