{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang JT"],"funding":["Chinese Academy of Sciences","Shanghai Key Laboratory of Embryo Original Diseases","Natural Science Foundation of China","Key Laboratory of Reproductive Genetics, Ministry of Education, Zhejiang University","National Key Research and Development Program of China"],"pagination":["2223-2239"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8887486"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["50(4)"],"pubmed_abstract":["N 6-Threonylcarbamoyladenosine (t6A) is a universal and pivotal tRNA modification. KEOPS in eukaryotes participates in its biogenesis, whose mutations are connected with Galloway-Mowat syndrome. However, the tRNA substrate selection mechanism by KEOPS and t6A modification function in mammalian cells remain unclear. Here, we confirmed that all ANN-decoding human cytoplasmic tRNAs harbor a t6A moiety. Using t6A modification systems from various eukaryotes, we proposed the possible coevolution of position 33 of initiator tRNAMet and modification enzymes. The role of the universal CCA end in t6A biogenesis varied among species. However, all KEOPSs critically depended on C32 and two base pairs in the D-stem. Knockdown of the catalytic subunit OSGEP in HEK293T cells had no effect on the steady-s"],"journal":["Nucleic acids research"],"pubmed_title":["Commonality and diversity in tRNA substrate recognition in t6A biogenesis by eukaryotic KEOPSs."],"pmcid":["PMC8887486"],"funding_grant_id":["2021YFA1300800","ZDFY2020-RG-0003","2021YFC2700903","31822015","91940302","XDB19010203","31670801","Shelab201904","32000847","81870896","2017YFA0504000","31870811","32000889"],"pubmed_authors":["Zhang W","Zhou L","Wang JT","Mao XL","Chen M","Zhou XL","Wang ED","Zhou JB"],"additional_accession":[]},"is_claimable":false,"name":"Commonality and diversity in tRNA substrate recognition in t6A biogenesis by eukaryotic KEOPSs.","description":"N 6-Threonylcarbamoyladenosine (t6A) is a universal and pivotal tRNA modification. KEOPS in eukaryotes participates in its biogenesis, whose mutations are connected with Galloway-Mowat syndrome. However, the tRNA substrate selection mechanism by KEOPS and t6A modification function in mammalian cells remain unclear. Here, we confirmed that all ANN-decoding human cytoplasmic tRNAs harbor a t6A moiety. Using t6A modification systems from various eukaryotes, we proposed the possible coevolution of position 33 of initiator tRNAMet and modification enzymes. The role of the universal CCA end in t6A biogenesis varied among species. However, all KEOPSs critically depended on C32 and two base pairs in the D-stem. Knockdown of the catalytic subunit OSGEP in HEK293T cells had no effect on the steady-s","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Feb","modification":"2026-05-31T04:01:17.007Z","creation":"2025-02-19T01:54:46.016Z"},"accession":"S-EPMC8887486","cross_references":{"pubmed":["35104889"],"doi":["10.1093/nar/gkac056"]}}