biostudies-literatureFu Qthe Natural Science Foundation of Hubei Provincial Department of Education17448069221076460https://www.ebi.ac.uk/biostudies/studies/S-EPMC8891909biostudies-literatureUnknown18Bone cancer pain (BCP) seriously affects the quality of life; however, due to its complex mechanism, the clinical treatment was unsatisfactory. Recent studies have showed several Rac-specific guanine nucleotide exchange factors (GEFs) that affect development and structure of neuronal processes play a vital role in the regulation of chronic pain. P-Rex2 is one of GEFs that regulate spine density, and the present study was performed to examine the effect of P-Rex2 on the development of BCP. Tumor cells implantation induced the mechanical hyperalgesia, which was accompanied by an increase in spinal protein P-Rex2, phosphorylated Rac1 (p-Rac1) and phosphorylated GluR1 (p-GluR1), and number of spines. Intrathecal injection a P-Rex2-targeting RNAi lentivirus relieved BCP and reduced the expressiMolecular painP-Rex2 mediation of synaptic plasticity contributes to bone cancer pain.PMC8891909B2020109Wan SFu QLi XLi YSu SHuang XXu XWu YfalseP-Rex2 mediation of synaptic plasticity contributes to bone cancer pain.Bone cancer pain (BCP) seriously affects the quality of life; however, due to its complex mechanism, the clinical treatment was unsatisfactory. Recent studies have showed several Rac-specific guanine nucleotide exchange factors (GEFs) that affect development and structure of neuronal processes play a vital role in the regulation of chronic pain. P-Rex2 is one of GEFs that regulate spine density, and the present study was performed to examine the effect of P-Rex2 on the development of BCP. Tumor cells implantation induced the mechanical hyperalgesia, which was accompanied by an increase in spinal protein P-Rex2, phosphorylated Rac1 (p-Rac1) and phosphorylated GluR1 (p-GluR1), and number of spines. Intrathecal injection a P-Rex2-targeting RNAi lentivirus relieved BCP and reduced the expressi2022-01-01T00:00:00Z2022 Jan-Dec2025-04-04T07:50:49.043Z2025-04-04T07:50:49.043ZS-EPMC88919093508394110.1177/17448069221076460