biostudies-literatureCarvalho SBFundação para a Ciência e a TecnologiaEuropean Commission805176https://www.ebi.ac.uk/biostudies/studies/S-EPMC8894879biostudies-literatureUnknown10Virus-like particles (VLPs) are excellent platforms for the development of influenza vaccine candidates. Nonetheless, their characterization is challenging due to VLPs' unique biophysical and biochemical properties. To cope with such complexity, multiple analytical techniques have been developed to date (e.g., single-particle analysis, thermal stability, or quantification assays), most of which are rarely used or have been successfully demonstrated for being applicable for virus particle characterization. In this study, several biophysical and biochemical methods have been evaluated for thorough characterization of monovalent and pentavalent influenza VLPs from diverse groups (A and B) and subtypes (H1 and H3) produced in insect cells using the baculovirus expression vector system (IC-BEVSFrontiers in bioengineering and biotechnologyBioanalytics for Influenza Virus-Like Particle Characterization and Process Monitoring.PMC8894879FP7-HEALTH-2013-INNOVATION−1, GA n. 602640SFRH/BD/52302/2013 SFRH/BPD/121558/2016 IF/01704/2014 EXPL/BBB-BIO/1541/2013 IF/01704/2014/CP1229/CT0001 UIDB/04462/2020Sousa MFQSilva RJSPeixoto CAlves PMRoldao ACarrondo MJTCarvalho SBfalseBioanalytics for Influenza Virus-Like Particle Characterization and Process Monitoring.Virus-like particles (VLPs) are excellent platforms for the development of influenza vaccine candidates. Nonetheless, their characterization is challenging due to VLPs' unique biophysical and biochemical properties. To cope with such complexity, multiple analytical techniques have been developed to date (e.g., single-particle analysis, thermal stability, or quantification assays), most of which are rarely used or have been successfully demonstrated for being applicable for virus particle characterization. In this study, several biophysical and biochemical methods have been evaluated for thorough characterization of monovalent and pentavalent influenza VLPs from diverse groups (A and B) and subtypes (H1 and H3) produced in insect cells using the baculovirus expression vector system (IC-BEVS2022-01-01T00:00:00Z20222025-06-01T02:24:26.562Z2024-11-14T20:05:58.186ZS-EPMC88948793525212810.3389/fbioe.2022.805176