{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Peck JV"],"funding":["NCI NIH HHS","Core Facility Advocacy Committee and Office of Research Technologies, UNC Chapel Hill School of Medicine","National Cancer Institute of the National Institutes of Health"],"pagination":["243-252"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8895008"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(Pt 2)"],"pubmed_abstract":["Limitations to successful single-particle cryo-electron microscopy (cryo-EM) projects include stable sample generation, production of quality cryo-EM grids with randomly oriented particles embedded in thin vitreous ice and access to microscope time. To address the limitation of microscope time, methodologies to more efficiently collect data on a 200 keV Talos Arctica cryo-transmission electron microscope at speeds as fast as 720 movies per hour (∼17 000 per day) were tested. In this study, key parameters were explored to increase data collection speed including: (1) using the beam-image shift method to acquire multiple images per stage position, (2) employing UltrAufoil TEM grids with R0.6/1 hole spacing, (3) collecting hardware-binned data and (4) adjusting the image shift delay factor in"],"journal":["IUCrJ"],"pubmed_title":["High-speed high-resolution data collection on a 200 keV cryo-TEM."],"pmcid":["PMC8895008"],"funding_grant_id":["P30CA016086","P30 CA016086"],"pubmed_authors":["Strauss JD","Peck JV","Fay JF"],"additional_accession":[]},"is_claimable":false,"name":"High-speed high-resolution data collection on a 200 keV cryo-TEM.","description":"Limitations to successful single-particle cryo-electron microscopy (cryo-EM) projects include stable sample generation, production of quality cryo-EM grids with randomly oriented particles embedded in thin vitreous ice and access to microscope time. To address the limitation of microscope time, methodologies to more efficiently collect data on a 200 keV Talos Arctica cryo-transmission electron microscope at speeds as fast as 720 movies per hour (∼17 000 per day) were tested. In this study, key parameters were explored to increase data collection speed including: (1) using the beam-image shift method to acquire multiple images per stage position, (2) employing UltrAufoil TEM grids with R0.6/1 hole spacing, (3) collecting hardware-binned data and (4) adjusting the image shift delay factor in","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2026-05-09T04:16:54.23Z","creation":"2025-02-19T00:56:24.35Z"},"accession":"S-EPMC8895008","cross_references":{"pubmed":["35371504"],"doi":["10.1107/S2052252522000069"]}}