biostudies-literatureHe MTianjin Science and Technology ProgramNatural Science Foundation of Tianjin CityMinistry of Science and Technology of the People&apos;s Republic of ChinaNational Natural Science Foundation of China806837https://www.ebi.ac.uk/biostudies/studies/S-EPMC8895481biostudies-literatureUnknown13Several approaches to expand human hematopoietic stem cells (hHSCs) clinically along with retainable capability of multipotential differentiation have been reported, but only a few have advanced to evaluation in clinical trials, which limits the application of HSC-based therapy. Here we show a phthalide derivative, Levistilide A (LA), can serve as a promising molecule to expand functional human umbilical cord blood (UCB) HSCs <i>ex vivo</i>. An in-house screen identified LA out of nine natural products as an outstanding candidate for hHSCs expansion. Additionally, our data indicated that LA treatment not only increased the numbers of phenotype-defined HSCs, but also enhanced their colony formation ability. Xenotransplantation assays showed that LA treatment could maintain unaffected engrafFrontiers in pharmacologyLevistilide A Promotes Expansion of Human Umbilical Cord Blood Hematopoietic Stem Cells by Enhancing Antioxidant Activity.PMC889548192068204 81870083 81970105 81872764 8190345620JCQNJC0208018ZXXYSY000102017YFA0104900 2016YFA0100600Wang CYang MZhang HHe MXu HZhang QZhang WLiu XLi YDing YZhang YGao YXu SLiu GfalseLevistilide A Promotes Expansion of Human Umbilical Cord Blood Hematopoietic Stem Cells by Enhancing Antioxidant Activity.Several approaches to expand human hematopoietic stem cells (hHSCs) clinically along with retainable capability of multipotential differentiation have been reported, but only a few have advanced to evaluation in clinical trials, which limits the application of HSC-based therapy. Here we show a phthalide derivative, Levistilide A (LA), can serve as a promising molecule to expand functional human umbilical cord blood (UCB) HSCs <i>ex vivo</i>. An in-house screen identified LA out of nine natural products as an outstanding candidate for hHSCs expansion. Additionally, our data indicated that LA treatment not only increased the numbers of phenotype-defined HSCs, but also enhanced their colony formation ability. Xenotransplantation assays showed that LA treatment could maintain unaffected engraf2022-01-01T00:00:00Z20222025-04-04T08:57:28.652Z2025-04-04T08:57:28.652ZS-EPMC88954813525055810.3389/fphar.2022.806837