biostudies-literatureWang XKey Program of China National Natural Science FundsGeneral Program of China National Natural Science FundsNatural Science Foundation of Shaanxi Province23https://www.ebi.ac.uk/biostudies/studies/S-EPMC8895872biostudies-literatureUnknown12(1)<h4>Objective</h4>The aim of this study was to investigate the pro-angiogenic potential of olfactory ensheathing cells (OECs) activated by curcumin (CCM) and lipopolysaccharide (LPS) and the possible underlying mechanisms.<h4>Methods</h4>Vascular endothelial cells or tissues were cultured and treated with conditioned medium (CM) extracted from activated OECs activated through the addition of LPS and CCM or unactivated controls. Concomitantly, the pro-angiogenic potential of OECs was assessed in vitro by aortic ring sprouting assay, endothelial wound healing assay, CCK-8 assay, and tube formation assay. Subsequently, the OECs were co-cultured with endothelial cells to evaluate their promoting effect on endothelial cell proliferation and migration following a mechanical scratch. Moreover, thCell & bioscienceThe promoting effects of activated olfactory ensheathing cells on angiogenesis after spinal cord injury through the PI3K/Akt pathway.PMC88958728177235781830077813714112020JM-686Yang HFan HLi JTian FChen ZZhang YJiang CWang XWang ZHao DfalseThe promoting effects of activated olfactory ensheathing cells on angiogenesis after spinal cord injury through the PI3K/Akt pathway.<h4>Objective</h4>The aim of this study was to investigate the pro-angiogenic potential of olfactory ensheathing cells (OECs) activated by curcumin (CCM) and lipopolysaccharide (LPS) and the possible underlying mechanisms.<h4>Methods</h4>Vascular endothelial cells or tissues were cultured and treated with conditioned medium (CM) extracted from activated OECs activated through the addition of LPS and CCM or unactivated controls. Concomitantly, the pro-angiogenic potential of OECs was assessed in vitro by aortic ring sprouting assay, endothelial wound healing assay, CCK-8 assay, and tube formation assay. Subsequently, the OECs were co-cultured with endothelial cells to evaluate their promoting effect on endothelial cell proliferation and migration following a mechanical scratch. Moreover, th2022-01-01T00:00:00Z2022 Mar2025-04-25T22:26:49.294Z2025-04-06T09:02:54.446ZS-EPMC88958723524624410.1186/s13578-022-00765-y