{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Guo H"],"funding":["American Cancer Society","Centers of Biomedical Research Excellence","National Institute of Diabetes and Digestive and Kidney Diseases","Cystic Fibrosis Foundation","National Institute of General Medical Sciences"],"pagination":["e69082"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8896829"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11"],"pubmed_abstract":["Apical constriction driven by actin and non-muscle myosin II (actomyosin) provides a well-conserved mechanism to mediate epithelial folding. It remains unclear how contractile forces near the apical surface of a cell sheet drive out-of-the-plane bending of the sheet and whether myosin contractility is required throughout folding. By optogenetic-mediated acute inhibition of actomyosin, we find that during Drosophila mesoderm invagination, actomyosin contractility is critical to prevent tissue relaxation during the early, 'priming' stage of folding but is dispensable for the actual folding step after the tissue passes through a stereotyped transitional configuration. This binary response suggests that Drosophila mesoderm is mechanically bistable during gastrulation. Computer modeling analysi"],"journal":["eLife"],"pubmed_title":["Optogenetic inhibition of actomyosin reveals mechanical bistability of the mesoderm epithelium during Drosophila mesoderm invagination."],"pmcid":["PMC8896829"],"funding_grant_id":["P20-GM113132","STANTO15R0","ESI-MIRA R35GM128745","P30-DK117469","#IRG -82-003-33"],"pubmed_authors":["Swan M","Guo H","He B"],"additional_accession":[]},"is_claimable":false,"name":"Optogenetic inhibition of actomyosin reveals mechanical bistability of the mesoderm epithelium during Drosophila mesoderm invagination.","description":"Apical constriction driven by actin and non-muscle myosin II (actomyosin) provides a well-conserved mechanism to mediate epithelial folding. It remains unclear how contractile forces near the apical surface of a cell sheet drive out-of-the-plane bending of the sheet and whether myosin contractility is required throughout folding. By optogenetic-mediated acute inhibition of actomyosin, we find that during Drosophila mesoderm invagination, actomyosin contractility is critical to prevent tissue relaxation during the early, 'priming' stage of folding but is dispensable for the actual folding step after the tissue passes through a stereotyped transitional configuration. This binary response suggests that Drosophila mesoderm is mechanically bistable during gastrulation. Computer modeling analysi","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Feb","modification":"2025-04-22T10:02:55.113Z","creation":"2025-04-05T23:23:08.648Z"},"accession":"S-EPMC8896829","cross_references":{"pubmed":["35195065"],"doi":["10.7554/eLife.69082"]}}