biostudies-literatureZheng LFok Ying Tong Education FoundationNational Natural Science Foundation of China209https://www.ebi.ac.uk/biostudies/studies/S-EPMC8897501biostudies-literatureUnknown13(3)Mechanotransduction sensing of tissue architecture and cellular microenvironment is a fundamental regulator of cell fate, including cancer. Meanwhile, long noncoding RNAs (lncRNAs) play multifunctions during cancer development and treatment. However, the link between lncRNAs and cellular mechanotransduction in the context of cancer progression has not yet been elucidated. In this study, using atomic force microscopy (AFM), we find that ionizing radiation reduces tumor stiffness. Ionizing radiation-induced lncRNA CRYBG3 can blunt YAP/TAZ activity through interference with mechanotransduction, resulting in the inhibition of cell proliferation, invasion, and metastasis of lung cancer cells. In vivo, we found that loss of lncRNA CRYBG3 could power the tumor initiation and metastasis ability, bCell death & diseaseIonizing radiation-induced long noncoding RNA CRYBG3 regulates YAP/TAZ through mechanotransduction.PMC889750117101731971165 and 82173465Chang LYang NPei HLuo CDong SJi MZhang WLi XZhang ZZheng LHei TKZhou GShu YWang XLi BWang YfalseIonizing radiation-induced long noncoding RNA CRYBG3 regulates YAP/TAZ through mechanotransduction.Mechanotransduction sensing of tissue architecture and cellular microenvironment is a fundamental regulator of cell fate, including cancer. Meanwhile, long noncoding RNAs (lncRNAs) play multifunctions during cancer development and treatment. However, the link between lncRNAs and cellular mechanotransduction in the context of cancer progression has not yet been elucidated. In this study, using atomic force microscopy (AFM), we find that ionizing radiation reduces tumor stiffness. Ionizing radiation-induced lncRNA CRYBG3 can blunt YAP/TAZ activity through interference with mechanotransduction, resulting in the inhibition of cell proliferation, invasion, and metastasis of lung cancer cells. In vivo, we found that loss of lncRNA CRYBG3 could power the tumor initiation and metastasis ability, b2022-01-01T00:00:00Z2022 Mar2025-04-26T18:28:18.846Z2025-04-06T15:50:19.904ZS-EPMC88975013524651110.1038/s41419-022-04650-x