biostudies-literatureYang WNIA NIH HHSBrightFocus FoundationNCI NIH HHSNational Institute on Aging353-361https://www.ebi.ac.uk/biostudies/studies/S-EPMC8900146biostudies-literatureUnknown31(4)The AMP-activated protein kinase (AMPK) is a molecular sensor to help maintain cellular energy homeostasis. AMPK is a heterotrimeric complex and its enzymatic catalytic subunit includes two isoforms: α1 and α2. Dysregulation of AMPK signaling is linked to neuronal diseases characterized with cognitive impairments. Emerging evidence also suggest isoform-specific roles of AMPK in the brain. AMPK regulates protein synthesis, which is critical for memory formation and neuronal plasticity. However, the consequence of altering AMPK activity on the translation of specific proteins in the brain is unknown. Here, we use unbiased mass spectrometry-based proteomics approach to analyze protein profile alterations in hippocampus and prefrontal cortex of transgenic mice in which the genes for the two AMHippocampusProtein expression alteration in hippocampus upon genetic repression of AMPKα isoforms.PMC8900146R01 AG056622R01 CA172115A2017457SP30 CA012197R01 CA131231R01 AG055581Yang WSun PLee JWang XWu DMa TZhou XFurdui CMfalseProtein expression alteration in hippocampus upon genetic repression of AMPKα isoforms.The AMP-activated protein kinase (AMPK) is a molecular sensor to help maintain cellular energy homeostasis. AMPK is a heterotrimeric complex and its enzymatic catalytic subunit includes two isoforms: α1 and α2. Dysregulation of AMPK signaling is linked to neuronal diseases characterized with cognitive impairments. Emerging evidence also suggest isoform-specific roles of AMPK in the brain. AMPK regulates protein synthesis, which is critical for memory formation and neuronal plasticity. However, the consequence of altering AMPK activity on the translation of specific proteins in the brain is unknown. Here, we use unbiased mass spectrometry-based proteomics approach to analyze protein profile alterations in hippocampus and prefrontal cortex of transgenic mice in which the genes for the two AM2021-01-01T00:00:00Z2021 Apr2026-06-01T02:32:41.008Z2024-10-16T14:57:01.04ZS-EPMC89001463349273210.1002/hipo.23305