biostudies-literatureUnknown6(2)Brouns SLN<h4>Background</h4>Low plasma levels of protein C or protein S are associated with venous thromboembolism rather than myocardial infarction. The high coagulant activity in patients with thrombophilia with a (familial) defect in protein C or S is explained by defective protein C activation, involving thrombomodulin and protein S. This causes increased plasmatic thrombin generation.<h4>Objective</h4>Assess the role of platelets in the thrombus- and fibrin-forming potential in patients with familial protein C or protein S deficiency under high-shear flow conditions.<h4>Patients/methods</h4>Whole blood from 23 patients and 15 control subjects was perfused over six glycoprotein VI-dependent microspot surfaces. By real-time multicolor microscopic imaging, kinetics of platelet thrombus and fibrinResearch and practice in thrombosis and haemostasise12678https://www.ebi.ac.uk/biostudies/studies/S-EPMC8900581biostudies-literatureProtein C or Protein S deficiency associates with paradoxically impaired platelet-dependent thrombus and fibrin formation under flow.PMC8900581van Oerle RPerrella GKuijpers MJESpiezia Lvan Geffen JPSimioni PTullemans BMEBrouns SLNCampello ESpronk HMHBulato Cvan der Meijden PEJHeemskerk JWMfalseProtein C or Protein S deficiency associates with paradoxically impaired platelet-dependent thrombus and fibrin formation under flow.<h4>Background</h4>Low plasma levels of protein C or protein S are associated with venous thromboembolism rather than myocardial infarction. The high coagulant activity in patients with thrombophilia with a (familial) defect in protein C or S is explained by defective protein C activation, involving thrombomodulin and protein S. This causes increased plasmatic thrombin generation.<h4>Objective</h4>Assess the role of platelets in the thrombus- and fibrin-forming potential in patients with familial protein C or protein S deficiency under high-shear flow conditions.<h4>Patients/methods</h4>Whole blood from 23 patients and 15 control subjects was perfused over six glycoprotein VI-dependent microspot surfaces. By real-time multicolor microscopic imaging, kinetics of platelet thrombus and fibrin2022-01-01T00:00:00Z2022 Feb2025-04-04T14:50:38.458Z2025-04-04T14:50:38.458ZS-EPMC89005813528477610.1002/rth2.12678